Human gut Bacteroides capture vitamin B12 via cell surface-exposed lipoproteins

ELife
Aaron G WexlerAndrew L Goodman

Abstract

Human gut Bacteroides use surface-exposed lipoproteins to bind and metabolize complex polysaccharides. Although vitamins and other nutrients are also essential for commensal fitness, much less is known about how commensal bacteria compete with each other or the host for these critical resources. Unlike in Escherichia coli, transport loci for vitamin B12 (cobalamin) and other corrinoids in human gut Bacteroides are replete with conserved genes encoding proteins whose functions are unknown. Here we report that one of these proteins, BtuG, is a surface-exposed lipoprotein that is essential for efficient B12 transport in B. thetaiotaomicron. BtuG binds B12 with femtomolar affinity and can remove B12 from intrinsic factor, a critical B12 transport protein in humans. Our studies suggest that Bacteroides use surface-exposed lipoproteins not only for capturing polysaccharides, but also to acquire key vitamins in the gut.

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Citations

Aug 26, 2020·Expert Review of Endocrinology & Metabolism·Dominic Oduro-DonkorThomas M Barber
May 13, 2020·BMC Medicine·Andrew J ForgieBenjamin P Willing
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Aug 15, 2021·Nature Reviews. Genetics·Julie Perreau, Nancy A Moran

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Methods Mentioned

BETA
biosensor
pull-down
tandem
pulls down
pull down
size-exclusion chromatography
chip
isothermal
surface plasmon resonance
lipidation

Software Mentioned

MAFFT
TOPP
Chimera
PyMOL
FigTree
CCP4
ClustalW
PDB2PQR
BlastP
BioEvaluation

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