Human Hair Outer Root Sheath Cells and Platelet-Lysis Exosomes Promote Hair Inductivity of Dermal Papilla Cell.

Tissue Engineering and Regenerative Medicine
Mohammad Ali NilforoushzadehEhsan Taghiabadi

Abstract

Hair loss is a prevalent medical problem in both men and women. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) during cell culture is the main factor in hair follicle morphogenesis and regeneration. The present study was conducted to compare the effects of different concentrations of human hair outer root sheath cell (HHORSC) and platelet lysis (PL) exosomes to maintain hair inductivity of the human dermal papilla cells (hDPCs). In this study, hDPCs and HHORSCs were isolated from healthy hair samples. Specific markers of hDPCs (versican, α-SMA) and HHORSCs (K15) were evaluated using flow cytometric and immunocytochemical techniques. The exosomes were isolated from HHORSCs and PL with ultracentrifugation technique. Western blot was used to detect specific markers of HHORSCs and PL exosomes. Particle size and distribution of the exosomes were analyzed by NanoSight dynamic light NanoSight Dynamic Light Scattering. Different methods such as proliferation test (MTS assay), migration test (Transwell assay) were used to evaluate the effects of different concentrations of exosomes (2,550,100 µg/ml) derived from HHORSC and PL on hDPCs. Expression of specific genes in the hair follicle inductivity, including AL...Continue Reading

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Citations

Dec 29, 2020·Journal of Cosmetic Dermatology·Mohammad Ali NilforoushzadehAmir Reza Hanifnia
Jun 27, 2021·Tissue Engineering and Regenerative Medicine·Jinyan WuNingwen Zhu
Jul 24, 2021·Tissue Engineering and Regenerative Medicine·Ji Yong Lee, Han-Soo Kim

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Methods Mentioned

BETA
flow cytometry
protein assay
dynamic light scattering
fluorescence microscopy
ELISA

Software Mentioned

Nanoparticle Tracking Analysis
ImageJ
HHORSCs
Exo
MINITAB

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