PMID: 7527723Jan 1, 1994Paper

Human hemoglobin conjugated to carboxylate dextran as a potential red blood cell substitute. I. Further physico-chemical characterization

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
P QuellecE Dellacherie

Abstract

In a previous paper, we described the preparation of a conjugate by reaction of benzene tetracarboxylate-substituted dextran (dex-BTC) with oxyhemoglobin. The first biological experiments carried out on animals showed that a solution of this type of product could be regarded as an oxygen-carrying fluid. The physico-chemical properties of this conjugate have been further characterized in view of its potential application in clinical experiments. Its molecular size distribution and the fraction of intramolecularly cross-linked hemoglobin were determined by elution on various types of chromatographic columns. Moreover the conjugate's oxygen-binding properties were studied in the presence of effectors and of Cl- ions, and the results showed that the allosteric site and the Val 1 alpha residues of conjugated hemoglobin were occupied by dex-BTC. Finally, the fractions obtained after gel filtration exhibited a similar P50 whatever their molecular sizes. So far, all the results obtained are compatible with the use of the conjugate solution as a blood substitute.

References

Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S R SnyderJ A Walder
Nov 28, 1983·Biochimica Et Biophysica Acta·E DellacherieC Vigneron

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Citations

Mar 2, 2016·International Journal of Biological Macromolecules·Aparna Banerjee, Rajib Bandopadhyay
Jun 9, 2004·Biochimica Et Biophysica Acta·Yiping JiaAbdu I Alayash
Sep 9, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Jun ZhangHong Zhou

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