PMID: 2495023Apr 3, 1989Paper

Human hepatic low-density lipoprotein receptors: associations of receptor activities in vitro with plasma lipid and apolipoprotein concentrations in vivo

Biochimica Et Biophysica Acta
M N Nanjee, N E Miller

Abstract

The relationships of plasma lipid and apolipoprotein (apo) concentrations to hepatic low-density lipoprotein (LDL) receptor activity were examined in 21 subjects (16 females, 5 males), who were undergoing laparotomy for non-neoplastic disease (cholecystectomy in 16). None had familial hypercholesterolemia, or renal, endocrine or hepatic disease. Ages were 37-77 years (mean, 58 years), plasma cholesterol concentrations 4.09-6.72 mmol/l (5.38) and plasma triacylglycerol concentrations 0.75-2.35 mmol/l (1.36). Receptor activity was quantified in vitro as the total saturable binding and EDTA-suppressible binding (representing apoB,E receptors) of 125I-labelled human LDL (15 micrograms protein/ml) by liver homogenate at 37 degrees C. There were no significant differences between men and women in 125I-labeled LDL binding. In the pooled data, EDTA-suppressible binding averaged 50 ng 125I-LDL protein/mg cell protein (S.D., 15). Total saturable binding averaged 2-fold greater (mean, 101 ng/mg; S.D., 32). Plasma cholesterol, LDL cholesterol and apoB concentrations were negative functions of both EDTA-suppressible binding and total saturable binding, but the correlations with EDTA-suppressible binding were stronger (cholesterol: r = -0.59...Continue Reading

References

Jan 2, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J ShepherdH G Morgan
Nov 1, 1977·Metabolism: Clinical and Experimental·J L Goldstein, M S Brown
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C PittmanD Steinberg
May 1, 1989·Journal of Medicinal Chemistry·A DonettiR Micheletti
Aug 1, 1987·The Journal of Clinical Investigation·F KremplerF Sandhofer
Feb 1, 1987·American Heart Journal·Y A KesäniemiT A Miettinen
Nov 1, 1985·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S M GrundyD W Bilheimer
Jun 1, 1972·The Journal of Clinical Investigation·T LangerR I Levy
Dec 20, 1973·Biochimica Et Biophysica Acta·S EisenbergF T Lindgren
Nov 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G Y WuR J Stockert
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P T KovanenJ L Goldstein
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T KitaJ L Goldstein
Nov 1, 1981·The Journal of Clinical Investigation·R W MahleyK H Weisgraber
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K Harders-SpengelA K Soutar
Jan 1, 1980·Annals of the New York Academy of Sciences·M S BrownJ L Goldstein
May 29, 1980·The New England Journal of Medicine·J ShepherdH G Morgan

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Citations

Oct 1, 1992·Free Radical Biology & Medicine·H EsterbauerG Jürgens
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M J RudlingB Angelin
Jul 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M F Kleinherenbrink-StinsD L Knook
Mar 10, 2004·The British Journal of Nutrition·Pujitha P de SilvaSukhinder Kaur Cheema
Oct 27, 2017·Drug Delivery·Xueqin Zhang, Gangliang Huang

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