PMID: 9537862Apr 16, 1998Paper

Human hepatic stellate cells express class I alcohol dehydrogenase and aldehyde dehydrogenase but not cytochrome P4502E1

Journal of Hepatology
A CasiniC Surrenti

Abstract

Alcohol dehydrogenase, cytochrome P4502E1 (CYP2E1), and aldehyde dehydrogenase are known to play an important role in alcohol metabolism in the liver. Although the ethanol oxidation pathways are mainly localized in hepatocytes, we examine whether human hepatic stellate cells might also metabolize ethanol and acetaldehyde. Hepatic stellate cells were isolated from normal human livers and exposed in vitro to 50 mmol/l ethanol or 85 micromol/l acetaldehyde for different periods of time. Alcohol dehydrogenase/aldehyde dehydrogenase activity and CYP2E1 protein expression were measured in hepatic stellate cells. Moreover, alcohol dehydrogenase and aldehyde dehydrogenase mRNA expression were evaluated in hepatic stellate cells. Exposure of hepatic stellate cells to ethanol for 24 h resulted in a 5-fold increase in cell alcohol dehydrogenase activity. The effect of ethanol on alcohol dehydrogenase activity was paralleled by a significant increase in the alcohol dehydrogenase mRNA expression in hepatic stellate cells. Acetaldehyde significantly increased the activity of high affinity aldehyde dehydrogenase in hepatic stellate cells, whereas ethanol was devoid of any effect. Acetaldehyde also induced high affinity aldehyde dehydrogenase ...Continue Reading

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Citations

Feb 15, 2012·Journal of Gastroenterology and Hepatology·Young-Sun Lee, Won-Il Jeong
May 13, 2009·The International Journal of Biochemistry & Cell Biology·Luigi AtzoriAndrea Perra
Mar 15, 2005·Clinics in Liver Disease·Charles S Lieber
Jun 14, 2016·Alcoholism, Clinical and Experimental Research·Elizabeth Brandon-WarnerLaura W Schrum
Mar 20, 2002·Addiction Biology·Hiroshi Matsumoto, Yuko Fukui
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N NietoA I Cederbaum
Dec 19, 2018·Basic & Clinical Pharmacology & Toxicology·Sael Casas-GrajalesPablo Muriel
Feb 20, 2010·Free Radical Research·Anna Maria BrunatiMaria Pia Rigobello
Oct 4, 2014·World Journal of Gastroenterology : WJG·Devanshi SethPaul S Haber
Feb 28, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Maya W HaakerJ Bernd Helms

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