Human hepatocellular carcinoma in a mouse model: assessment of tumor response to percutaneous ablation by using glyceraldehyde-3-phosphate dehydrogenase antagonists.

Radiology
Shanmugasundaram Ganapathy-KanniappanJean-François H Geschwind

Abstract

To characterize tumor response to percutaneous injection of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antagonists in a mouse model of human hepatocellular carcinoma (HCC). Animal experiments were approved by the Johns Hopkins University Animal Care and Use Committee. Luciferase (luc) gene-expressing Hep3B tumor-bearing athymic nude mice were randomly divided into four groups of six mice each. Tumor-specific GAPDH inhibition was achieved by using percutaneous injection of GAPDH antagonists-3-bromopyruvate (3-BrPA) or GAPDH-specific short hairpin RNA (shRNA). Tumor response to treatment was assessed by using bioluminescence imaging and analysis of GAPDH function and apoptotic markers (caspase-3, caspase-9, and positive staining for terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphospate nick end labeling). HCC samples from 34 patients were obtained from the Johns Hopkins tumor bank, as approved by the Institutional Review Board, for GAPDH expression analysis. Statistical analysis was performed by using a two-sample t test or Spearman rank correlation coefficient. In vitro, 3-BrPA affected Hep3B cell viability (half maximal inhibitory concentration = 0.15 mmol/L), and GAPDH shRNA suppressed (45.5%) colony ...Continue Reading

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Citations

Jul 23, 2013·BMC Research Notes·Rani KunjithapathamShanmugasundaram Ganapathy-Kanniappan
Oct 1, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao-lin ZhuMei-xia Zhang
Mar 16, 2013·Journal of Vascular and Interventional Radiology : JVIR·Manon BuijsJean-Francois H Geschwind
Nov 4, 2015·Biochemical and Biophysical Research Communications·Swathi KarthikeyanShanmugasundaram Ganapathy-Kanniappan
Oct 30, 2014·Scientific Reports·Rani KunjithapathamShanmugasundaram Ganapathy-Kanniappan
Sep 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gabriela Carrasco-TorresVerónica Rocío Vásquez-Garzón
Mar 4, 2015·Journal of Proteome Research·Rani KunjithapathamShanmugasundaram Ganapathy-Kanniappan
Jul 28, 2016·Journal of Bioenergetics and Biomembranes·J Azevedo-SilvaM Casal
Apr 8, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shanshan LiuKun Huang
Mar 9, 2018·Expert Opinion on Therapeutic Targets·Shanmugasundaram Ganapathy-Kanniappan
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Jan 22, 2020·Journal of Bioenergetics and Biomembranes·Somayeh YousefiAli Akbar Pourfathollah
Sep 12, 2012·Oncotarget·Shanmugasundaram Ganapathy-KanniappanJean-Francois Geschwind
Jul 8, 2020·Antioxidants & Redox Signaling·Jingwen JiangCanhua Huang
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Oct 23, 2020·Life Sciences·Majid GhanavatNajmaldin Saki
Nov 28, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Gabriela L OliveiraPaulo J Oliveira
Nov 12, 2021·Frontiers in Physiology·Himanshi PanditaShanmugasundaram Ganapathy-Kanniappan

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