Human herpesvirus 6 impairs differentiation of monocytes to dendritic cells

Experimental Hematology
Hironari NiiyaMasaki Yasukawa

Abstract

Monocyte-derived dendritic cells (DCs) play important roles in the immune response against infections and malignancies. Human herpesvirus 6 (HHV-6) infects monocytes and is reactivated in immunodeficient patients. To clarify the mechanisms of HHV-6-induced immunodeficiency, we investigated the effect of HHV-6 infection on differentiation of monocytes to DCs. Monocytes were inoculated with or without HHV-6 and then allowed to differentiate to myeloid DCs in culture medium containing granulocyte-macrophage colony-stimulating factor and interleukin (IL)-4. The expression of cell surface molecules on DCs and the capacity of the DCs for antigen capture were examined by flow cytometric analysis. Alteration of antigen-presenting capacity induced by HHV-6 infection was examined. The morphology of HHV-6-infected monocyte-derived DCs was distinctly different from that of the DCs derived from mock-infected monocytes. Although expression levels of DC-associated surface antigens, including CD80, CD83, and CD86, were significantly higher on HHV-6-infected monocyte-derived DCs than on DCs derived from mock-infected monocytes, antigen-presenting capacity was significantly lower in the former group. Addition of culture supernatant of HHV-6-infe...Continue Reading

References

Apr 1, 1992·Journal of Medical Microbiology·W L Irving
Jun 1, 1991·The Journal of General Virology·K KondoK Yamanishi
Jan 1, 1990·Journal of Clinical Laboratory Analysis·J LukaG Thiele
Nov 15, 1988·International Journal of Cancer. Journal International Du Cancer·D V AblashiR C Gallo
Jan 1, 1995·Acta Haematologica·K SugitaT Furukawa
Feb 1, 1995·Immunology Today·P Lusso, R C Gallo
Jun 1, 1993·The Journal of Infectious Diseases·R T HorvatB Chandran
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·L J Zhou, T F Tedder
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·J J SchnorrS Schneider-Schaulies
Sep 18, 1997·The Journal of Experimental Medicine·I GrosjeanD Kaiserlian
Sep 18, 1997·The Journal of Experimental Medicine·I Fugier-VivierC Rabourdin-Combe
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Jun 28, 2003·Blood·Alison SmithPaolo Lusso
Oct 31, 2003·Expert Reviews in Molecular Medicine·S DewhurstN van Loon
Nov 12, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I NordøyF Müller
Feb 27, 2004·British Journal of Haematology·Tetsushi Yoshikawa
Jan 18, 2005·Clinical Microbiology Reviews·Leen De BolleErik De Clercq

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.