Human herpesvirus-6 rep/U94 gene product has single-stranded DNA-binding activity

The Journal of General Virology
P DhepaksonK Yamanishi

Abstract

The characterization is reported of the human herpesvirus-6B (HHV-6B) rep/U94 gene, which is a homologue of the adeno-associated virus type 2 rep. In this study, a monoclonal antibody was produced against HHV-6B REP (anti-REP mAb). Immunofluorescence staining using the anti-REP mAb showed that REP was localized to the nucleus in HHV-6-infected MT4 cells. It was first detected at 24 h post-infection (p.i.) and accumulated to higher levels by 72 h p.i. REP may be expressed only at very low levels in HHV-6-infected cells: even when the late protein glycoprotein H was detected in nearly 90% of HHV-6-infected cells, REP was detected in only a small percentage of them. Western blot analysis showed that the anti-REP mAb recognized a 56-kDa polypeptide in HHV-6B-infected MT4 cells. Furthermore, the REP protein was shown to bind single-stranded DNA.

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Citations

Apr 5, 2011·Microbes and Infection·Jesse H Arbuckle, Peter G Medveczky
Nov 13, 2013·Microbiology and Immunology·Chyntia JasirwanYasuko Mori
Oct 28, 2014·Microbiology and Immunology·Chyntia JasirwanYasuko Mori
Feb 8, 2014·Virology·Mayuko HayashiYasuko Mori
Dec 3, 2014·Current Opinion in Virology·Benedikt B Kaufer, Louis Flamand
Dec 2, 2014·Current Opinion in Virology·Laurie T Krug, Philip E Pellett
Feb 15, 2013·PloS One·Akihiro ShimizuKazuhiro Kondo
Nov 3, 2014·Annual Review of Virology·Nikolaus OsterriederBenedikt B Kaufer
Nov 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Arnaldo CarusoDario Di Luca
Jul 24, 2015·Journal of Virology·Huamin Tang, Yasuko Mori
Apr 15, 2011·Uirusu·Huamin TangYasuko Mori
Jan 18, 2005·Clinical Microbiology Reviews·Leen De BolleErik De Clercq
Mar 8, 2019·Journal of Virology·Bochao WangYasuko Mori
Sep 7, 2018·Frontiers in Microbiology·Bhupesh K PrustyCarla T Toro
Sep 17, 2010·Journal of Virology·Guillaume Morissette, Louis Flamand
Dec 20, 2019·Journal of Virology·Bochao WangYasuko Mori
Apr 17, 2020·Journal of Virology·Jingjing MaHuamin Tang
Feb 16, 2006·The Journal of General Virology·Tomohiko SadaokaYasuko Mori
Jan 25, 2006·The Journal of General Virology·Honglan HuangYasuko Mori
Aug 23, 2020·Microbiology and Immunology·Anna Lystia PoetrantoYasuko Mori

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