Abstract
The quantitative evaluation of human herpesvirus 8 (HHV8) DNA is not well described in the clinical management of HHV8-related lymphoproliferative diseases. To evaluate and to compare HHV8 viral load in different blood compartments from patients with multicentric Castleman's disease (MCD), primary effusion lymphoma (PEL) and HHV8-associated solid lymphoma (SLY) and to establish which clinical sample would be preferable for HHV8 DNA testing. We assessed HHV8 DNA in plasma and peripheral blood mononuclear cells (PBMCs) paired samples from 7 PEL, 8 MCD, 2 SLY HIV+ patients at the diagnosis and during the course of the illness by using a real time PCR assay. HHV8 viremia was always detectable at diagnosis. HHV8 DNA levels were correlated in matched pairs of samples at diagnosis and during follow-up (Spearman correlation coefficient: r=0.83, p<0.001 and r=0.73, p<0.001, respectively). The performance characteristics of the PCR assay with both materials did not show disparity by the analysis of the receiver operating characteristic (ROC) curve (X(1)(2)=0.50; p=0.48). Plasma or PBMCs are both adequate samples for HHV8 DNA quantification and Real time PCR provides a reliable method to estimate viral replication in patients with HHV8-re...Continue Reading
References
Oct 1, 1978·Seminars in Nuclear Medicine·C E Metz
Sep 23, 1995·Lancet·D WhitbyA S Denton
Jun 30, 1998·Archives of Dermatology·P CattaniD Cerimele
Jan 29, 2000·Cancer·J IscovichR Sarid
Feb 5, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·H KatanoS Mori
Nov 4, 2000·AIDS·T B CampbellR T Schooley
Nov 24, 2001·Blood·M CorbellinoC Parravicini
Mar 16, 2002·Blood·Eric OksenhendlerVéronique Meignin
Sep 13, 2002·Journal of Medical Virology·Jian SongNorihiro Nishimoto
Apr 26, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas B CampbellLovemore Gwanzura
Oct 29, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Cecelia SimonelliUmberto Tirelli
Apr 20, 2004·AIDS·Dimitra BourbouliaChris Boshoff
Oct 19, 2004·The American Journal of Surgical Pathology·Amy ChadburnDaniel M Knowles
Apr 13, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cecilia SimonelliUmberto Tirelli
May 24, 2005·Cancer Letters·Maria Teresa BortolinPaolo De Paoli
Jul 5, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Emmanuelle BoulangerEric Oksenhendler
Dec 31, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Cecilia SimonelliAntonino Carbone
Citations
Sep 29, 2011·Transplantation·Ella J Ariza-Heredia, Raymund R Razonable
Jul 2, 2010·Infectious Agents and Cancer·Lynnette K TumwineStefano A Pileri
Sep 10, 2013·Best Practice & Research. Clinical Anaesthesiology·Malte BookFrank Stüber
Aug 6, 2014·Leukemia & Lymphoma·Musa AlzahraniHeather A Leitch
Jun 26, 2015·HIV Medicine·I-U HaqM Bower
Sep 3, 2013·European Journal of Haematology·Eric OksenhendlerLionel Galicier
Mar 31, 2016·Future Microbiology·Gilbert GreubKatia Jaton
Apr 23, 2011·Blood·Justin StebbingMark Bower
Aug 7, 2010·Blood·Mark Bower
Oct 15, 2011·International Journal of STD & AIDS·R SayerR F Miller
Mar 8, 2019·Clinical Transplantation·Rebecca Pellett MadanUNKNOWN AST Infectious Diseases Community of Practice
Dec 26, 2018·Transplantation Direct·Gabriel M CohenSarah Taimur
Aug 25, 2015·Oncotarget·Rosamaria TedeschiPaolo De Paoli