PMID: 6159646Sep 1, 1980Paper

Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity

Proceedings of the National Academy of Sciences of the United States of America
L Olsson, H S Kaplan

Abstract

We report the establishment of human-human hybridomas producing monoclonal antibody of predefined antigenic specificity. The U-266 human myeloma cell line was incubated in the presence of 8-azaguanine, and a rapidly growing, 8-azaguanine-resistant, hypoxanthine/amethopterin/thymidine (HAT) medium-sensitive mutant line, U-266AR1, was selected. These cells were fused with lymphoid cells from uninvolved spleens removed at staging laparotomy from patients with untreated Hodgkin's disease who had been previously sensitized to the chemical allergen 2,4-dinitrochlorobenzne. Hybrid cell cultures growing in HAT medium were screened for IgG production. Positive cultures were selected and their supernatants were tested in a solid-phase radioimmunoassay for reactivity with dinitrophenyl hapten coupled to bovine serum albumin. Cultures producing specific antibody were subcloned and expanded, and their antibody products were shown to be monoclonal by biosynthetic labeling and sodium dodecyl sulfate/polyacrylamide gel electrophoresis.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R Levy, J Dilley
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Aug 27, 1974·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C R Parish, J A Hayward

❮ Previous
Next ❯

Citations

Mar 1, 1990·Molecular Reproduction and Development·R ShalgiR Jones
Jan 1, 1983·Cancer Metastasis Reviews·L Olsson
Jan 1, 1996·Cytotechnology·T Lindl
Jan 1, 1983·Journal of Cancer Research and Clinical Oncology·J Zeuthen
Oct 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·I V Spirande
Jan 1, 1982·The Journal of Membrane Biology·U Zimmermann, J Vienken
Feb 1, 1985·European Journal of Clinical Microbiology·H Krakauer
Apr 29, 1998·Diseases of the Colon and Rectum·L Stocchi, H Nelson
Jan 1, 1984·Antonie van Leeuwenhoek·O T Schönherr, E H Houwink
Dec 1, 1981·In Vitro·R H Kennett
Dec 1, 1981·In Vitro·M D Scharff, S Roberts
Oct 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H MurakamiH Omura
Jan 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S HashizumeH Omura
Sep 1, 1982·Indian Journal of Pediatrics·A K Abbas
Sep 15, 1982·Cellular Immunology·S HochJ Schwaber
Jun 24, 1983·Journal of Immunological Methods·L OlssonB Jakobsen
May 23, 1985·Journal of Immunological Methods·B G TalbotD M Gibson
Jul 16, 1985·Journal of Immunological Methods·M C Glassy, C D Surh
May 1, 1986·Journal of Immunological Methods·W L CarrollR Levy
Nov 6, 1986·Journal of Immunological Methods·R L WassermanE S Thaxton
Aug 3, 1987·Journal of Immunological Methods·S R SamoilovichA J Macario
Dec 8, 1992·Journal of Immunological Methods·I ZanellaA Albertini
Nov 1, 1990·Veterinary Immunology and Immunopathology·A R GreenleeW C Davis
Aug 1, 1986·Journal of Virological Methods·M J RedmondD G Scraba
Apr 1, 1988·Immunology Today·K M Thompson
Mar 1, 1984·Human Immunology·J F SchwaberH Lazarus
Jul 1, 1985·Human Immunology·A DoyleB A Bradley
Dec 17, 1982·Biochimica Et Biophysica Acta·W J BallJ L Lessard
Dec 17, 1986·Biochimica Et Biophysica Acta·G FossatiG Parmiani
Jul 1, 1985·Journal of Ultrastructure Research·J C Herr, D R Spell
May 24, 2005·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S SiberilJ-L Teillaud
Dec 21, 2000·Journal of Immunological Methods·C F JessupH Zola

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D KozborJ C Roder
Proceedings of the National Academy of Sciences of the United States of America
N N TengH S Kaplan
Proceedings of the National Academy of Sciences of the United States of America
M C GlassyI Royston
© 2021 Meta ULC. All rights reserved