Human IgE mRNA expression by peripheral blood lymphocytes stimulated with interleukin 4 and pokeweed mitogen

European Journal of Immunology
G QiuB M Stadler

Abstract

Expression of human IgE mRNA by peripheral blood lymphocytes (PBL) and an IgE-producing myeloma cell line, U-266, was examined by Northern blot hybridization and compared with IgE levels in culture supernatants. A 2.35-kb IgE mRNA was detected in unstimulated atopic PBL and U-266 cells but not in normal PBL, and its levels correlated with IgE protein levels in the supernatant. Upon stimulation with interleukin 4, a new 1.75-kb transcript was revealed in both atopic and normal PBL but not in U-266 cells. Its expression did not correlate with IgE levels in the supernatant. Pokeweed mitogen also induced the expression of the 1.75-kb transcript without concomitant induction of IgE synthesis by normal PBL and even suppressed the spontaneous expression of the 2.35-kb transcript and IgE protein synthesis by atopic PBL. Interferon-gamma, which suppressed both the 2.35-kb transcript and IgE protein production, had no inhibitory effect on the 1.75-kb transcript. Expression of the 1.75-kb transcript was already high after 2 days of stimulation and peaked around day 4. The length of the transcript is smaller than that of mRNA coding for secreted human IgG and IgA and contains all four C epsilon exon sequences, suggesting it might be a trun...Continue Reading

References

Feb 1, 1986·Analytical Biochemistry·R M Amasino
Jan 1, 1986·Molecular Biology Reports·E W Khandjian
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J StavnezerE Severinson
Jun 1, 1989·European Journal of Immunology·J F GauchatB M Stadler
Feb 1, 1989·The Journal of Experimental Medicine·T MacKenzie, H M Dosch
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G ThyphronitisF D Finkelman
Mar 1, 1989·Journal of Clinical Immunology·D Vercelli, R S Geha
Jan 1, 1987·Methods in Enzymology·F J CalzoneE H Davidson
Apr 1, 1986·The Journal of Allergy and Clinical Immunology·J G Massicot, K Ishizaka
Jul 1, 1986·Journal of Clinical Immunology·D Y Leung, R S Geha
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PèneJ Banchereau
Sep 11, 1985·Nucleic Acids Research·E T Schenborn, R C Mierendorf
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y SakoyamaT Honjo
Nov 1, 1988·European Journal of Immunology·X D YangB M Stadler
Jul 1, 1988·European Journal of Immunology·S Bergstedt-LindqvistE Severinson
Dec 1, 1988·The Journal of Experimental Medicine·P RothmanF W Alt
Feb 1, 1988·Immunological Reviews·C M SnapperW E Paul
Aug 1, 1986·Allergy·J M Knutti-MüllerL de Weck
Jul 25, 1984·Journal of Molecular Biology·M L SteenU Pettersson
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N MigoneL L Cavalli-Sforza
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Mar 25, 1983·Journal of Immunological Methods·A B EisenbreyG J Gleich
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H HisajimaT Honjo
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H KikutaniJ Stavnezer
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y NishidaT Honjo

❮ Previous
Next ❯

Citations

Dec 1, 1991·Immunology·K J TurnerM W Beilharz
Aug 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·J IrschA Radbruch
Jul 1, 1991·British Journal of Haematology·R E Callard
Aug 1, 1997·Allergy·E Jensen-JarolimB M Stadler
Feb 1, 1996·Experimental Dermatology·I AebischerB M Stadler
Sep 1, 1993·European Journal of Immunology·K Kotowicz, R E Callard
Sep 1, 1994·European Journal of Immunology·M R StämpfliB M Stadler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.