PMID: 2109100May 1, 1990Paper

Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells

Journal of Virology
P A AshornBernard Moss

Abstract

Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant vaccinia virus that can infect and promote transient expression of full-length CD4 in mammalian cells. This virus, together with another vaccinia recombinant encoding biologically active HIV envelope glycoprotein gp160, allowed us to study CD4/gp160-mediated cell-cell fusion in a wide variety of human and nonhuman cells in the absence of other HIV proteins. By using syncytium formation assays in which a single cell type expressed both CD4 and gp160, we demonstrated membrane fusion in lymphoid and nonlymphoid human cells but not in any of the 23 tested nonhuman cell types, derived from African green monkey, baboon, rabbit, hamster, rat, or mouse. However, in mixing experiments with one cell type expressing CD4 and the other cell type expressing gp160, all of these nonhuman cells could form CD4/gp160-mediated syncytia when mixed with human cells; in 20 of 23 cases, membrane fusion occurred only if the CD4 molecule was...Continue Reading

References

Jul 1, 1987·Molecular and Cellular Biology·T R FuerstB Moss
Jan 1, 1980·Journal of Immunological Methods·E C Butcher, I L Weissman

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Citations

Sep 1, 1995·AIDS Research and Human Retroviruses·M M Guo, J E Hildreth
Oct 26, 1999·Viral Immunology·A N VzorovR W Compans
Jul 1, 1997·Expert Opinion on Investigational Drugs·D R Pakianathan
Jan 1, 1990·Annals of the New York Academy of Sciences·R Datema
Nov 1, 1991·Scandinavian Journal of Immunology·J M AlonsoC Martínez
Sep 1, 1992·AIDS Research and Human Retroviruses·H GoldingR Blumenthal
Jan 1, 1992·AIDS Research and Human Retroviruses·M O McClureR A Weiss
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·D Unutmaz, D R Littman
Apr 1, 1992·AIDS Research and Human Retroviruses·J P Moore, P J Klasse
Oct 13, 1995·The Journal of Biological Chemistry·E O Freed, M A Martin
Dec 16, 1991·FEBS Letters·J R Harris
Feb 17, 1997·The Journal of Experimental Medicine·R I ConnorN R Landau
Dec 30, 1998·Archives of Pharmacal Research·H Choe
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z LuR W Doms
Jun 25, 2015·Frontiers in Immunology·Edward A Berger
Apr 30, 2002·Current Opinion in Infectious Diseases·N Cammack
Sep 1, 1996·AIDS Research and Human Retroviruses·W A PaxtonJ P Moore
Feb 2, 2018·Journal of Leukocyte Biology·Sarah B Joseph, Ronald Swanstrom
Jun 6, 2000·Advanced Drug Delivery Reviews·P J KlasseM Marsh

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