Human Immunodeficiency Virus (HIV)-Antibody Repertoire Estimates Reservoir Size and Time of Antiretroviral Therapy Initiation in Virally Suppressed Perinatally HIV-Infected Children

Journal of the Pediatric Infectious Diseases Society
Salvatore RoccaEPIICAL Consortium

Abstract

Assays to estimate human immunodeficiency virus (HIV) reservoir size require large amounts of blood, which represents a drawback especially in pediatric settings. We investigated whether HIV-antibody repertoire could estimate the viral reservoir size. Moreover, we assessed the magnitude of HIV-antibody response as a predictor of time of antiretroviral therapy (ART) initiation. Human immunodeficiency virus-antibody responses to 10 different viral proteins were evaluated by HIV Western blot (WB) kit and a WB score was assigned to each patient. Patients were classified in 2 subgroups based on the timing of ART initiation (early treated [ET], 0-24 weeks and late treated [LT], >24 weeks). Human immunodeficiency virus-deoxyribonucleic acid (DNA) was quantified using real-time quantitative polymerase chain reaction on total peripheral blood mononuclear cells. Logistic regression and principal component analysis were built on these data to test the ability of WB score to predict the expected value of HIV-DNA and the timing of ART initiation. Sixty-nine perinatally HIV-infected children were evaluated. Reduced HIV-specific antibody responses and lower size of HIV-DNA were observed in ET compared with LT patients (P < .001 and P = .02, r...Continue Reading

References

Oct 15, 2011·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Jill C Stoltzfus
Nov 30, 2013·The Journal of Infectious Diseases·Peter D BurbeloSteven G Deeks
Nov 22, 2014·Current Opinion in HIV and AIDS·Jintanat AnanworanichNicolas Chomont
Mar 10, 2015·Trends in Microbiology·Katherine M BrunerRobert F Siliciano
Jun 13, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marta Martínez-BonetJavier Martinez-Picado
Apr 13, 2017·Current HIV/AIDS Reports·Radwa R Sharaf, Jonathan Z Li
May 13, 2017·The Journal of Infectious Diseases·Sheila M KeatingMichael P Busch
Jun 14, 2017·AIDS·Caroline FosterUNKNOWN CHERUB Investigators

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Citations

Jan 1, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefano RinaldiSavita Pahwa
Jan 10, 2020·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Thanyawee PuthanakitUNKNOWN RV475/HIV-NAT209 Study Group
Jul 18, 2020·Journal of the Pediatric Infectious Diseases Society·Caroline FosterPablo Rojo
Sep 1, 2021·The Journal of Antimicrobial Chemotherapy·Laura Tarancón-DiezFrancesc Vidal

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