PMID: 7545240Oct 1, 1995Paper

Human immunodeficiency virus type 1 can use different tRNAs as primers for reverse transcription but selectively maintains a primer binding site complementary to tRNA(3Lys)

Journal of Virology
J K WakefieldCasey D Morrow

Abstract

The initiation of human immunodeficiency virus type 1 (HIV-1) reverse transcription occurs at a site in the viral RNA genome which is designated the primer-binding site (PBS). The HIV-1 PBS is an 18-nucleotide sequence that is complementary to the 3'-terminal 18 nucleotides of tRNA(3Lys), which is used as the primer for reverse transcription. All HIV-1 isolates sequenced to date contain a PBS complementary to tRNA(3Lys), suggesting that other cellular tRNAs might not function as primers for reverse transcription. To investigate this possibility, we have substituted the HIV-1 PBS with sequences predicted to be complementary to the 3'-terminal nucleotides of tRNA(1,2Lys), tRNA(Ile), and tRNA(His), which previous studies have identified to be packaged into HIV-1 virions along with tRNA(3Lys). We demonstrate that infectious viruses which utilized tRNA(1,2Lys), tRNA(Ile), and tRNA(His) in reverse transcription can be recovered. However, the appearances of viruses with PBSs complementary to these alternate tRNAs were delayed compared with the wild type. After extended in vitro culture, viruses containing the PBSs complementary to these different tRNAs reverted back to the wild-type PBS complementary to tRNA3(Lys). Furthermore, only t...Continue Reading

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Citations

Feb 10, 1997·AIDS Research and Human Retroviruses·Y LiC D Morrow
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·B Chan, K Musier-Forsyth
Mar 7, 2001·European Journal of Biochemistry·J F CabodevillaJ J Martínez-Irujo
Jan 15, 2005·AIDS Research and Human Retroviruses·Matthew J RendaStephen Dewhurst
Oct 30, 2007·FEBS Letters·Elisabetta Viani Puglisi, Joseph D Puglisi
Aug 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel WolfStephen P Goff
Jun 19, 2009·Expert Opinion on Biological Therapy·Virendra N PandeyBinay Chaubey
Apr 10, 1999·The Journal of Biological Chemistry·M GötteH Heumann
Oct 6, 1997·The Journal of Biological Chemistry·C J GregoireE P Loret
Nov 27, 2009·The Journal of General Virology·A TuiskunenS Plumet
Jan 1, 2010·Viruses·Catherine IselRoland Marquet
Dec 10, 1998·Nature Structural Biology·E V Puglisi, J D Puglisi
Jul 30, 2003·Journal of Virology·Nathan J KellyCasey D Morrow
Apr 6, 2019·Retrovirology·Christine M FennesseyBrandon F Keele
Apr 15, 2000·The Journal of Biological Chemistry·J M LanchyR Marquet
Feb 1, 2019·The Journal of Biological Chemistry·Danni Jin, Karin Musier-Forsyth
Sep 12, 2020·Current Opinion in Structural Biology·Miri KrupkinElisabetta Viani Puglisi
Dec 31, 2020·International Journal of Molecular Sciences·Thomas GremmingerXiao Heng

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