Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells

Journal of Virology
Diane M P LawrenceEugene O Major

Abstract

Although cells of monocytic lineage are the primary source of human immunodeficiency virus type 1 (HIV-1) in the brain, other cell types in the central nervous system, including astrocytes, can harbor a latent or persistent HIV-1 infection. In the present study, we examined whether immature, multipotential human brain-derived progenitor cells (nestin positive) are also permissive for infection. When exposed to IIIB and NL4-3 strains of HIV-1, progenitor cells and progenitor-derived astrocytes became infected, with peak p24 levels of 100 to 500 pg/ml at 3 to 6 days postinfection. After 10 days, virus production was undetectable but could be stimulated by the addition of tumor necrosis factor alpha (TNF-alpha). To bypass limitations to receptor entry, we compared the fate of infection in these cell populations by transfection with the infectious HIV-1 clone, pNL4-3. Again, transfected progenitors and astrocytes produced virus for 7 days but diminished to low levels beyond 8 days posttransfection. During the nonproductive phase, TNF-alpha stimulated virus production from progenitors as late as 5 weeks posttransfection. Astrocytes produced 5- to 20-fold more infectious virus (27 ng of p24/10(6) cells) than progenitors at the peak o...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G PoliA S Fauci
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T M FolksA S Fauci
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C A WileyM B Oldstone
Jun 20, 1995·Virology·F EnsoliC J Thiele
Jan 1, 1994·Advances in Neuroimmunology·L R SharerB M Blumberg
Sep 1, 1994·Journal of Leukocyte Biology·H E GendelmanH S Nottet
May 1, 1995·Journal of the International Neuropsychological Society : JINS·R K HeatonR J Ellis
Aug 5, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D M Simpson
Mar 16, 2000·Seminars in Neurology·A Nath
Feb 13, 2001·Neurology·N SacktorUNKNOWN Multicenter AIDS Cohort Study
Aug 28, 2001·AIDS Research and Human Retroviruses·B Schweighardt, W J Atwood
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jutta K NeuenburgWolfgang Schlote
Feb 8, 2003·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pankaj SethRadha K Maheshwari
Mar 19, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shane QuiterioBrian Wigdahl
May 6, 2003·Annals of Neurology·Conrad A MessamEugene O Major

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Citations

Nov 2, 2005·Neurotoxicity Research·K F HauserP E Knapp
Nov 2, 2005·Neurotoxicity Research·P Seth, E O Major
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Joan W BermanWalter Royal
Nov 27, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Alessia BachisItalo Mocchetti
Feb 3, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kathryn E Medders, Marcus Kaul
May 12, 2005·Virus Research·Susanne Kramer-HämmerleRuth Brack-Werner
Dec 16, 2005·Cell Research·J Roberto TrujilloRobert C Gallo
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Juliane HaedickeMojgan H Naghavi
Sep 10, 2008·Journal of Neurovirology·Leslie CrewsEliezer Masliah
Jun 27, 2006·AIDS Research and Human Retroviruses·M Sarah HillEdward B Stephens
Mar 3, 2007·DNA and Cell Biology·Xiaodong ChengZahida Parveen
Dec 20, 2007·AIDS·Ina RothenaignerRuth Brack-Werner
Dec 15, 2005·Journal of Virology·Deborah Carroll-Anzinger, Lena Al-Harthi
Mar 14, 2007·Blood·J Bruce SundstromAftab A Ansari
Jan 14, 2011·Retrovirology·Timo Z Nazari-ShaftiReza Izadpanah
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Marcus Kaul
Apr 29, 2009·International Journal of Molecular Sciences·Leslie CrewsEliezer Masliah
Apr 26, 2013·Future Virology·Jane Kovalevich, Dianne Langford
Jan 29, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Shaily MalikPankaj Seth
Aug 20, 2014·Cellular and Molecular Life Sciences : CMLS·Darren Ferrell, Brian Giunta
Oct 17, 2009·International Journal of Biological Sciences·Ann Marie NelsonYan-gao Man
Nov 10, 2013·Immunologic Research·Rebeca Geffin, Micheline McCarthy
Jun 11, 2008·Journal of Neurochemistry·Sulagna Das, Anirban Basu
Dec 2, 2008·The Journal of Pathology·G Yadirgi, S Marino
Dec 19, 2006·Journal of Neurochemistry·Kurt F HauserPamela E Knapp
Nov 22, 2005·Neurobiology of Disease·Gareth Jones, Christopher Power
Sep 16, 2014·Neuroscience Research·Ashok ChauhanArwa Mujahid Abdullah
Nov 5, 2014·Journal of Neurovirology·Avindra Nath

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