PMID: 9445076Jan 28, 1998Paper

Human immunodeficiency virus type 1 matrix protein interacts with cellular protein HO3

Journal of Virology
J Lama, D Trono

Abstract

The matrix (MA) protein of human immunodeficiency virus type 1 (HIV-1) plays a critical role in virion morphogenesis and fulfills important functions during the early steps of infection. In an effort to identify cellular partners of MA, a Saccharomyces cerevisiae two-hybrid screen was utilized. A specific interaction between MA and HO3, a putative histidyl-tRNA synthetase, was demonstrated in this system. HO3-specific mRNA was detected in several tissues relevant for HIV infection, such as spleen, thymus, and peripheral blood lymphocytes, as well as in a number of T-lymphoid-cell lines. The binding of MA to HO3 was confirmed in transfected cells by coimmunoprecipitation. This interaction was abrogated by replacing two lysine residues at positions 26 and 27 of MA by threonine (MA(KK27TT)). HO3 localized both to the cytoplasm and to the nucleus of acutely transfected 293T cells. When overexpressed in HIV-1-producing cells, HO3 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of MA. Correspondingly, overexpression of HO3 in virus producer cells enhanced the infectivity of wild-type but not MA(KK27AA) HIV-1 particles. The stimulating effect of HO3 was independent from the presence of E...Continue Reading

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Citations

Jan 9, 1999·The Journal of Biological Chemistry·R PeytaviP Boulanger
Apr 14, 2005·Journal of Virology·Cagan GurerJeremy Luban
Jul 19, 2012·PloS One·Cameron J SchweitzerMichael Belshan
Nov 1, 2002·Reviews in Medical Virology·David E Ott
Oct 17, 2014·Journal of Virology·Pascal JalaguierMichel J Tremblay
Sep 12, 2015·Proteomics. Clinical Applications·Yan LiMichael Belshan
Sep 13, 2015·Virology Journal·Valerie Le SageAndrew J Mouland
May 25, 2004·The Journal of Biological Chemistry·Bénédicte HemonnotLaurence Briant
Sep 6, 2000·Current Opinion in Microbiology·S R Thompson, P Sarnow
May 22, 2002·Biochimica Et Biophysica Acta·Vaea Richard de SoultraitMichel Fournier
Jun 12, 2002·Biochemical and Biophysical Research Communications·Terrance P O'Hanlon, Frederick W Miller
Aug 14, 2021·Cell Host & Microbe·Charles Bou-NaderJinwei Zhang
Mar 21, 2006·Retrovirology·Alan W CochraneAndrew J Mouland

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