PMID: 7539505Jul 1, 1995Paper

Human immunodeficiency virus type 1 Nef increases the efficiency of reverse transcription in the infected cell

Journal of Virology
O SchwartzJ M Heard

Abstract

We have analyzed the replication of Nef+ and Nef- isogenic human immunodeficiency virus in CEM, HUT78, MT4 lymphoid, and U937 monocytic cell lines. At each passage of infected cells, we have assessed the relative infectivity of the virus particles released in culture media by measuring the number of infections units per nanogram of p24 protein. Values appeared to be 3- to 10-fold higher for the Nef+ virus than for the Nef- number The positive effect of Nef was observed regardless of the cell line, the multiplicity of infection, and the number of virus replication cycles achieved. We showed, by using cells expressing glycosylphosphatidylinositol-linked CD4, that the enhancement of virion infectivity could be dissociated from the down-regulation of cell surface CD4 also induced by Nef. The gp120-to-p24 ratio and the RNA content of virus particles produced in the presence or in the absence of Nef were equivalent. Virions bound to cell surface CD4 receptors with equal efficiencies. Equivalent reverse transcriptase activities were measured both on exogenous substrate and on particle genomic RNAs. In contrast, reverse transcription in infected cells generated 5- to 10-fold less DNA when the virions were produced in the absence of Nef...Continue Reading

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Citations

Feb 26, 2000·Annual Review of Genetics·H TangF Wong-Staal
Nov 9, 2000·Journal of Virology·V K AroraJ V Garcia
Jun 7, 2002·Journal of Virology·Fatima SerhanGhislaine Duisit
Jun 7, 2002·Journal of Virology·Tatyana DorfmanHeinrich G Göttlinger
Oct 25, 2003·Blood·Arnaud MorisOlivier Schwartz
Nov 3, 2006·Journal of Virology·Marion SourisseauOlivier Schwartz
Feb 22, 2008·Journal of Virology·Keith R Jerome
Feb 16, 2011·The Journal of Biological Chemistry·Paul J VorsterYuntao Wu
Apr 8, 2014·The Journal of Biological Chemistry·Miriam M GeistOliver T Fackler
Jun 7, 2014·Frontiers in Microbiology·Stéphane Basmaciogullari, Massimo Pizzato
Sep 30, 2015·Nature·Yoshiko UsamiHeinrich G Göttlinger
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Mar 21, 2000·Medicinal Research Reviews·H JonckheereE De Clercq
Jul 1, 1999·Reviews in Medical Virology·V Piguet, D Trono
Apr 1, 1996·Nature Structural Biology·M Stevenson
Sep 16, 1999·The Journal of Clinical Investigation·D FenardA Doglio
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·P Stumptner-CuveletteP Benaroch
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Apr 14, 2004·The Journal of Experimental Medicine·Sylvain CardinaudAndré Habel
Jul 5, 2005·Expert Opinion on Therapeutic Targets·S H ColemanJ C Guatelli
Mar 30, 2004·Journal of Virology·Ellen KrautkrämerOliver T Fackler
Aug 22, 2008·Journal of Virology·Massimo PizzatoHeinrich G Göttlinger
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Massimo Pizzato

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