PMID: 9188585Jul 1, 1997Paper

Human immunodeficiency virus type 1 nucleocapsid protein promotes efficient strand transfer and specific viral DNA synthesis by inhibiting TAR-dependent self-priming from minus-strand strong-stop DNA

Journal of Virology
J GuoJ G Levin

Abstract

During the first strand transfer in reverse transcription, minus-strand strong-stop DNA [(-) SSDNA] is annealed to the 3' end of the acceptor RNA in a reaction mediated by base-pairing between terminal repeat sequences in the RNA and their complement in the DNA. The large stem-loop structure in the repeat region known as TAR could interfere with this annealing reaction. We have developed an in vitro human immunodeficiency virus type 1 (HIV-1) system to investigate the effect of TAR on strand transfer. Mutational analysis demonstrates that the presence of TAR in the donor and acceptor templates inhibits strand transfer and is correlated with extensive synthesis of heterogeneous DNAs formed by self-priming from (-) SSDNA. These DNAs are not precursors to the transfer product. Interestingly, products of self-priming are not detected in HIV-1 endogenous reactions; this suggests that virions contain a component which prevents self-priming. Our results show that the viral nucleocapsid protein (NC), which can destabilize secondary structures, drastically reduces self-priming and dramatically increases the efficiency of strand transfer. In addition, the data suggest that the ability to eliminate self-priming is a general property of NC...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V GopalakrishnanS J Benkovic
Jan 1, 1992·Annual Review of Cell Biology·J M Whitcomb, S H Hughes
Jul 1, 1992·Journal of Virology·T B Fu, J Taylor
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H De RocquignyJ L Darlix
Nov 30, 1990·Science·W S Hu, H M Temin
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J GorelickA Rein
Dec 1, 1973·Journal of Virology·E Fleissner, E Tress
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M ChenT S Papas
Aug 1, 1981·The Journal of General Virology·K W Sykora, K Moelling
Dec 8, 1995·Journal of Molecular Biology·J L DarlixB P Roques
Jan 1, 1995·Biochimie·R MarquetB Ehresmann
Jul 11, 1995·Nucleic Acids Research·M Lapadat-TapolskyJ L Darlix
Sep 8, 1995·The Journal of Biological Chemistry·D Herschlag
Oct 6, 1995·Journal of Molecular Biology·V TanchouJ L Darlix
Oct 1, 1993·Science·Z TsuchihashiD Herschlag
Sep 1, 1993·Journal of Virology·W Fu, A Rein
Feb 1, 1993·Protein Science : a Publication of the Protein Society·F Dib-HajjD P Giedroc

❮ Previous
Next ❯

Citations

Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DruillennecM C Fournié-Zaluski
Jul 25, 2003·The Journal of Biological Chemistry·Yan ChenRobert A Bambara
Oct 19, 2007·Nucleic Acids Research·Yasumasa IwataniJudith G Levin
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Oct 29, 2011·Cellular and Molecular Life Sciences : CMLS·Bin XueVladimir N Uversky
May 23, 1998·The Journal of Biological Chemistry·C D Kelleher, J J Champoux
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Negroni, H Buc
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·M C WilliamsV A Bloomfield
May 29, 2003·The Journal of General Virology·Wataru AkahataMasanori Hayami
Jun 20, 2006·The Journal of Biological Chemistry·Min SongRobert A Bambara
Aug 8, 2008·Journal of Virology·Kristen M Stewart-MaynardKarin Musier-Forsyth
Dec 13, 2007·The Journal of Biological Chemistry·Min SongRobert A Bambara
Mar 30, 2011·RNA Biology·Dorota Piekna-Przybylska, Robert A Bambara
Dec 17, 2010·RNA Biology·Judith G LevinKarin Musier-Forsyth
Sep 25, 1999·The Journal of Biological Chemistry·M WisniewskiR A Bambara
Jan 20, 2005·The Journal of Biological Chemistry·Yan ChenRobert A Bambara
Dec 25, 2002·The Journal of Biological Chemistry·Yan ChenRobert A Bambara
May 23, 2002·The Journal of Biological Chemistry·Michele WisniewskiRobert A Bambara
Dec 8, 2009·Nature Structural & Molecular Biology·Dorota Piekna-PrzybylskaRobert A Bambara
Jan 6, 2007·The Journal of Biological Chemistry·Lu GaoRobert A Bambara
Jul 7, 2001·The Journal of Biological Chemistry·J J DeStefanoM J Fitzgerald-Heath

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.