PMID: 8972565Jan 1, 1996Paper

Human immunodeficiency virus type 1 Rev-dependent effects on the late gene expression of recombinant human adenovirus

Virus Genes
R D Williams, K N Leppard

Abstract

Both human adenovirus type 5 (Ad5) and human immunodeficiency virus (HIV-1) encode functions (E1b 55K/E4 orf6 and Rev respectively) which are needed for the efficient cytoplasmic accumulation of late viral mRNAs and which act post-transcriptionally. The similarity between these two regulatory systems was explored by constructing recombinant Ad5 carrying either one or both of the two components of the HIV-1 system (Rev and its RNA target sequence, RRE) and analysing the effects on Ad5 gene expression. In the presence of a functional Ad5 regulatory system, the HIV-1 system had no detectable effect on Ad5 gene expression. When the Ad5 system was rendered inoperative by mutation, the HIV-1 Rev/RRE system brought about a modest increase in the cytoplasmic accumulation of RRE-containing mRNAs. This was not sufficient to complement effectively the defect due to the Ad5 mutation. These data suggest that HIV-1 Rev acts on a similar pool of nuclear RNA to that targeted by Ad5 E1b 55K/E4 orf6 but functions inefficiently in the situation studied here. Effects were also observed on the accumulation of certain Ad5 mRNAs from which the RRE had been removed by splicing. No change was observed in the balance of production of differentially spli...Continue Reading

References

Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Dec 7, 1989·Nature·M L Zapp, M R Green
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B K FelberG N Pavlakis
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C A RosenW A Haseltine
Jan 1, 1995·Current Topics in Microbiology and Immunology·M J ImperialeK N Leppard
Jul 1, 1994·Genes & Development·B E Meyer, M H Malim
Apr 1, 1993·The Journal of General Virology·K N Leppard

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Citations

Aug 22, 2008·Journal of Public Health·Saulat JahanShamandy Abdul Rahim Hamed
Oct 10, 1998·Journal of Virology·J J Horridge, K N Leppard

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