PMID: 8970952Dec 1, 1996Paper

Human immunodeficiency virus type 1 Rev function requires continued synthesis of its target mRNA

Journal of Virology
S Iacampo, A W Cochrane

Abstract

Synthesis of human immunodeficiency virus structural proteins is dependent on expression of the virus-encoded Rev protein due to the constitutive nuclear sequestration of mRNAs coding for the structural proteins. The pathway by which Rev, through interaction with the Rev-responsive element (RRE) within the mRNA, achieves export of the mRNA remains unclear. To probe the mechanism by which Rev induces nuclear export of its target mRNAs, the effect of inhibiting mRNA synthesis on the function of Rev was examined. Two approaches to address this issue were pursued: (i) the use of general transcription inhibitors such as 5,6-dichlorobenzimidazole riboside (DRB) and actinomycin D, and (ii) the more selective modulation of target gene transcription permitted by the use of a tetracycline-regulated promoter. Addition of either DRB or actinomycin D inhibited Rev action despite the presence of significant quantities of the target mRNA throughout the course of drug treatment. Furthermore, prolonged DRB treatment was found to improve rather than diminish the induction observed. Subsequent analysis using the tetracycline-modulated promoter demonstrated that Rev function was dependent on the transcription rate of the target mRNA and independen...Continue Reading

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Citations

Mar 9, 2002·Journal of Virology·Andrew W DangelKathleen Boris-Lawrie
Apr 30, 2010·Journal of Virology·Maik BlissenbachKlaus Uberla
Jan 1, 2012·Biology·Anna Kula, Alessandro Marcello
Jan 19, 1999·Annual Review of Microbiology·V W Pollard, M H Malim
Apr 5, 2013·PLoS Pathogens·Raymond W WongAlan Cochrane
May 31, 2018·Nucleic Acids Research·Gatikrushna SinghKathleen Boris-Lawrie
May 18, 1999·Archives of Biochemistry and Biophysics·T J Hope
Mar 21, 2006·Retrovirology·Alan W CochraneAndrew J Mouland
Jun 23, 2004·RNA·Meredith McLarenAlan Cochrane

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