PMID: 7539924Jun 6, 1995Paper

Human immunodeficiency virus type 1 reverse transcriptase: enhancement of activity by interaction with cellular topoisomerase I

Proceedings of the National Academy of Sciences of the United States of America
Hidehiro TakahashiWilliam W Hall

Abstract

A number of studies have suggested that topoisomerase I (topo I) activity may be important in human immunodeficiency virus type 1 (HIV-1) replication. Specifically it has been reported that purified virus particles have topo I activity and that inhibitors of this enzyme can inhibit virus replication in vitro. We have investigated a possible association of HIV-1 gag proteins with topo I activity. We found that whereas the gag-encoded proteins by themselves do not have activity, the nucleocapsid protein p15 can interact with and enhance the activity of cellular topo I. Furthermore it could be demonstrated that topo I markedly enhanced HIV-1 reverse transcriptase activity in vitro and that this could be inhibited by the topo I-specific inhibitor camptothecin. The findings suggest that cellular topo I plays an important role in the reverse transcription of HIV-1 RNA and that the recruitment of this enzyme may be an important step in virus replication.

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Citations

Mar 21, 2000·Medicinal Research Reviews·H JonckheereE De Clercq
Jan 1, 1996·Journal of Biomedical Science·P. Pantazis
Jan 7, 2004·Biochemical and Biophysical Research Communications·Hidehiro TakahashiTakeshi Kurata
Jun 8, 2002·Biochemical and Biophysical Research Communications·Hidehiro TakahashiTakeshi Kurata
Sep 25, 2002·Biochemical and Biophysical Research Communications·Hidehiro TakahashiTakeshi Kurata
Sep 28, 1998·Biochimica Et Biophysica Acta·Y PommierD Strumberg
Sep 28, 1998·Biochimica Et Biophysica Acta·C H TakimotoS G Arbuck
Jun 6, 1998·Biochimie·Y Pommier
Mar 17, 1997·The EMBO Journal·D HarrichR B Gaynor
May 10, 2013·Journal of Virology·Kei TakahashiYoshihiro Kawaoka
Dec 19, 2013·Retrovirology·Charline GiroudLaurence Briant
Nov 20, 2013·AIDS Research and Therapy·Gero HütterHarald Klüter
Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yuko ShoyaHidehiro Takahashi
Feb 24, 2004·Microbiology and Immunology·Tomonori UenoHidehiro Takahashi
Nov 12, 2009·Microbiology and Immunology·Yoshinori KitagawaHidehiro Takahashi
Mar 7, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·U VanhoeferY M Rustum
Aug 15, 1998·Biochemical and Biophysical Research Communications·M KawamuraA Adachi

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