PMID: 8970945Dec 1, 1996Paper

Human immunodeficiency virus type 1 Vif does not influence expression or virion incorporation of gag-, pol-, and env-encoded proteins

Journal of Virology
R A FouchierM H Malim

Abstract

The Vif protein of human immunodeficiency virus type 1 is required for productive replication in peripheral blood lymphocytes and a limited number of immortalized T-lymphoid lines (nonpermissive cells). In contrast, Vif is fully dispensable for virus replication in other T-cell lines (permissive cells). Because the infection phenotype of released virions is determined by producer cells and by the presence of Vif in those cells, we have analyzed the protein contents of purified viral particles in an attempt to define compositional differences that could explain the infection phenotype. Surprisingly, we were unable to discern any Vif- or cell-type-dependent quantitative or qualitative difference in the Gag, Pol, and Env proteins of virions or virus-producing cells that correlates with virus infectivity. We were, however, able to demonstrate that Vif itself is present in virions and, using semiquantitative Western blotting (immunoblotting), that there is an average of 30 to 80 molecules of Vif incorporated into each virion. Importantly, parallel analyses of total lysates of the producer cells revealed that the cell-associated expression levels of Vif are close to those of the Gag proteins. Given the dramatically higher abundance o...Continue Reading

References

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Citations

Oct 29, 1998·The Journal of Biological Chemistry·X Yang, D Gabuzda
Feb 23, 2000·Journal of Virology·U ChatterjiJ H Elder
Nov 9, 2000·Journal of Virology·A Ohagen, D Gabuzda
May 26, 2004·The Journal of Biological Chemistry·Shan CenLawrence Kleiman
Jul 5, 2007·The Journal of Biological Chemistry·Serena BernacchiRoland Marquet
Jun 3, 2016·PLoS Pathogens·Mohamed Abdel-MohsenSatish K Pillai
Feb 26, 2000·Annual Review of Genetics·H TangF Wong-Staal
Dec 10, 1998·Nature Medicine·J H SimonM H Malim
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Keiko SakaiMichael J Lenardo
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Aug 3, 2005·AIDS Research and Human Retroviruses·Kristine M RoseDavid Kabat
Nov 29, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael H Malim
Jul 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·April J SchumacherReuben S Harris
Sep 11, 2014·Frontiers in Microbiology·Yuqing FengLinda Chelico
Sep 29, 2015·Veterinary Sciences·Deepanwita BoseYahia Chebloune
Apr 18, 2003·AIDS·Suryaram Gummuluru, Michael Emerman
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Dec 1, 2004·Annual Review of Genetics·Stephen P Goff
Jul 5, 2016·Nature Microbiology·Yang LuoMark A Muesing
Oct 19, 2007·Nucleic Acids Research·Yasumasa IwataniJudith G Levin

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