PMID: 9188609Jul 1, 1997Paper

Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition

Journal of Virology
M D MillerF D Bushman

Abstract

We have investigated the organization and function of human immunodeficiency virus type 1 (HIV-1) preintegration complexes (PICs), the large nucleoprotein particles that carry out cDNA integration in vivo. PICs can be isolated from HIV-1-infected cells, and such particles are capable of carrying out integration reactions in vitro. We find that although the PICs are large, the cDNA must be condensed to fit into the measured volume. The ends of the cDNA are probably linked by a protein bridge, since coordinated joining of the two ends is not disrupted by cleaving the cDNA internally with a restriction enzyme. cDNA ends in PICs were protected from digestion by added exonucleases, probably due to binding of proteins. The intervening cDNA, in contrast, was susceptible to attack by endonucleases. Previous work has established that the virus-encoded integrase protein is present in PICs, and we have reported recently that the host protein HMG I(Y) is also present. Here we report that the viral matrix and reverse transcriptase (RT) proteins also cofractionated with PICs through several steps whereas capsid and nucleocapsid proteins dissociated. These data support a model of PIC organization in which the cDNA is condensed in a partially ...Continue Reading

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Citations

Nov 9, 2000·Journal of Virology·K E Yoder, F D Bushman
Jan 11, 2001·Journal of Virology·M P ShermanW C Greene
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·T PushkarskyM Bukrinsky
Jul 2, 2002·Nature Medicine·Warner C Greene, B Matija Peterlin
May 22, 2002·Journal of Virology·Susanne WernerBirgitta M Wöhrl
Aug 7, 2002·Journal of Virology·Theodore C PiersonRobert F Siliciano
Nov 6, 2002·The Journal of Cell Biology·David McDonaldThomas J Hope
Dec 28, 2002·Journal of Virology·Kui GaoFrederic Bushman
May 14, 2003·Journal of Virology·Roger Chiu, Duane P Grandgenett
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Aug 3, 2005·Journal of Computer-aided Molecular Design·Rajeshri G KarkiMarc C Nicklaus
Nov 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christina Marchetti Bradley, Robert Craigie
Jan 18, 2006·Journal of Virology·Loraine Jarrosson-WuillemeAndrea Cimarelli
Jan 30, 2008·Antimicrobial Agents and Chemotherapy·Isabelle MaletAnne-Geneviève Marcelin
Nov 6, 2009·Journal of Virology·Lise RivièreAndrea Cimarelli
Oct 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephen HarePeter Cherepanov
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Oct 7, 2011·Journal of Virology·Eric C LogueNathaniel R Landau
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Sep 5, 2014·Macromolecular Bioscience·Maarten Danial, Harm-Anton Klok
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Jan 27, 2014·Biomedicines·Aaron Shaw, Kenneth Cornetta
Apr 26, 2019·Proceedings of the National Academy of Sciences of the United States of America·Junpeng YanJacek Skowronski
Jan 17, 2020·Journal of Virology·Irena Zurnic BönischZeger Debyser
Jan 9, 1999·The Journal of Biological Chemistry·R PeytaviP Boulanger
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·B SherryM Bukrinsky

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