Human induced pluripotent stem cell-derived neurons improve motor asymmetry in a 6-hydroxydopamine-induced rat model of Parkinson's disease

Cytotherapy
Fabin HanGuangyao Li

Abstract

Since human embryonic stem cells and human fetal neural stem cells have immune rejection and ethical issues, recent advancements in induced pluripotent stem cells (iPS cells) provide new possibilities to study autologous cell therapy for Parkinson's disease (PD). We isolated human skin fibroblasts from normal individuals and patients with PD; we generated iPS cells by transfecting these human skin fibroblasts with retroviral reprogramming factors of OCT4, SOX2, KLF4 and c-MYC and induced iPS cells to differentiate neural stem cells (NSCs) and then into neurons and dopamine neurons in vitro. We found that iPS cell-derived NSC transplant into the striatum of the 6-hydroxydopamine (OHDA)-induced PD rats improved their functional defects of rotational asymmetry at 4, 8, 12 and 16 weeks after transplantation. iPS cell-derived NSCs were found to survive and integrate into the brain of transplanted PD rats and differentiated into neurons, including dopamine neurons in vivo. Transplantation of iPS cell-derived NSCs has therapeutic potential for PD. Our study provided experimental proof for future clinical application of iPS cells in cell-based treatment of PD.

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Citations

Feb 18, 2016·Stem Cells International·Ji-Feng KangJi-Feng Guo
Mar 31, 2016·Neuroscience and Biobehavioral Reviews·Tetsuya AsakawaYing Xia
Dec 23, 2015·Neural Regeneration Research·Robin E White, Denis S Barry
Sep 5, 2015·Translational Neurodegeneration·Fabin HanQingfa Chen
Jul 16, 2015·Human Gene Therapy·Andrii DomanskyiMikko Airavaara
Jan 30, 2016·The International Journal of Neuroscience·Bangfu ZhuBing Song
Aug 16, 2016·Journal of Neural Transmission·Song LiWeidong Le
Oct 10, 2018·Frontiers in Cellular Neuroscience·Ziqian ChengRanji Cui
Feb 13, 2019·International Journal of Molecular Sciences·Michiel Van BulckJose A Morales-Garcia
Jul 6, 2019·Frontiers in Neuroscience·Jiangnan MaAnmu Xie
Mar 3, 2021·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Milad Ahani-NahayatiAli Hassanzadeh
Feb 6, 2018·Cytotherapy·Javad Amini MahabadiHossein Nikzad
Oct 5, 2021·Frontiers in Cell and Developmental Biology·Julianna LilienbergLászló Homolya

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