Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection

Stem Cell Research & Therapy
Danping HongYuehong Wu

Abstract

Induced pluripotent stem cells (iPS) represent an innovative source for the standardized in vitro generation of macrophages (Mφ). Mφ show great promise in disease pathogenesis, particularly tuberculosis. However, there is no information about human iPS-derived (hiPS) macrophages (hiPS-Mφ) in response to tuberculosis infection. In the present study, macrophages derived from hiPS were established via embryoid body (EB) formation by using feeder-free culture conditions, and the human monocyte cell line THP-1 (THP-1-Mφ) was used as control. iPS-Mφ were characterized by using morphology, Giemsa staining, nonspecific esterase staining (α-NAE), phagocytosis, and surface phenotype. Additionally, after treatment with Bacillus Calmette-Guérin (BCG) for 24 h, cell apoptosis was detected by using an Annexin V-FITC Apoptosis Detection assay. The production of nitric oxide (NO), expression of tumor necrosis factor alpha (TNF-α), activity of apoptosis-related protein cysteine-3 (Caspase-3) and expression of B-cell lymphoma-2 (Bcl-2) were analyzed. With respect to morphology, surface phenotype, and function, the iPS-Mφ closely resembled their counterparts generated in vitro from a human monocyte cell line. iPS-Mφ exhibited the typically morpho...Continue Reading

References

Jun 26, 2004·Journal of Leukocyte Biology·Robert D Stout, Jill Suttles
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eiji EsashiAtsushi Miyajima
Dec 23, 2008·Cell Stem Cell·Jing LiaoLei Xiao
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Toshihiko EzashiR Michael Roberts
Aug 15, 2009·Human Molecular Genetics·Chunliang LiYing Jin
Apr 7, 2010·Stem Cell Research·Yuehong WuPeter J Hornsby
Jul 14, 2010·Cell Stem Cell·Judith StaerkRudolf Jaenisch
Dec 2, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amal KambalJoseph S Anderson
Oct 11, 2011·Nature Reviews. Immunology·Ajay ChawlaY P Sharon Goh
Jan 17, 2012·Nature Cell Biology·Tim AhfeldtChad A Cowan
May 7, 2014·Oncoimmunology·Satoru SenjuYasuharu Nishimura
Jul 19, 2014·Immunity·Slava EpelmanGwendalyn J Randolph
Oct 24, 2014·Frontiers in Immunology·Guillermo Arango Duque, Albert Descoteaux
Dec 4, 2014·Nature·Elisa Gomez PerdigueroHans-Reimer Rodewald
Nov 7, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Rajat M GuptaKiran Musunuru
Jul 10, 2016·Tissue Engineering. Part C, Methods·Jaichandran SivalingamSteve Kah-Weng Oh
Jun 20, 2017·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Mania AckermannNico Lachmann

❮ Previous
Next ❯

Citations

Jul 11, 2020·International Journal of Molecular Sciences·Simon GutbierChristoph Patsch
Aug 9, 2020·Cancers·Thomas J GardnerDavid Scheinberg
Apr 2, 2021·Cellular Microbiology·Enrica Pellegrino, Maximiliano G Gutierrez

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA
FCS
FACS
fluorescence microscopy
flow cytometry
ELISA
Assay

Software Mentioned

SPSS
FlowJo
Adobe Photoshop

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.