Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes

Journal of Translational Medicine
Yu Jin ParkHyun Ok Kim

Abstract

The in vitro production of mature human red blood cells (RBCs) from induced pluripotent stem cells (iPSCs) has been the focus of research to meet the high demand for blood transfusions. However, limitations like high costs and technological requirements restrict the use of RBCs produced by iPSC differentiation to specific circumstances, such as for patients with rare blood types or alloimmunized patients. In this study, we developed a detailed protocol for the generation of iPSC lines derived from peripheral blood of donors with O D-positive blood and rare blood types (D-and Jr(a-)) and subsequent erythroid differentiation. Mononuclear cells separated from the peripheral blood of O D-positive and rare blood type donors were cultured to produce and expand erythroid progenitors and reprogrammed into iPSCs. A 31-day serum-free, xeno-free erythroid differentiation protocol was used to generate reticulocytes. The stability of iPSC lines was confirmed with chromosomal analysis and RT-PCR. Morphology and cell counts were determined by microscopy observations and flow cytometry. Cells from all donors were successfully used to generate iPSC lines, which were differentiated into erythroid precursors without any apparent chromosomal mutat...Continue Reading

References

Dec 28, 2004·Nature Biotechnology·Marie-Catherine GiarratanaLuc Douay
Apr 30, 2008·JAMA : the Journal of the American Medical Association·Charles NatansonSidney M Wolfe
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Sep 21, 2010·Transfusion·Andreas GreinacherWolfgang Hoffmann
Mar 26, 2011·Stem Cells and Development·Jessica DiasIgor I Slukvin
May 28, 2013·Lancet·Lorna M Williamson, Dana V Devine
Dec 24, 2013·Stem Cells Translational Medicine·Siddharth ShahLinzhao Cheng
Feb 12, 2014·Journal of Visualized Experiments : JoVE·Bradley HoweFern Tsien
Mar 19, 2014·Trends in Biotechnology·Abdu I Alayash
Mar 20, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·David ColliganJerard Seghatchian
May 13, 2014·Blood Cancer Journal·D FocosiM Pistello
Aug 6, 2014·The Journal of Experimental Medicine·Rakesh VermaDon M Wojchowski
Nov 20, 2014·Journal of Visualized Experiments : JoVE·Jesse J HubbardAravind Ramakrishnan
Dec 24, 2014·Indian Journal of Anaesthesia·Ranadhir MitraGirija Prasad Rath
Apr 15, 2015·Journal of the American Chemical Society·David H KwanStephen G Withers
Nov 4, 2015·Current Protocols in Immunology·Warren Strober
Jan 29, 2016·Stem Cells Translational Medicine·Daniele Focosi, Mauro Pistello
Mar 22, 2016·British Journal of Haematology·Jennifer EasterbrookLesley M Forrester
Apr 5, 2016·Hematology/oncology Clinics of North America·David J RobertsImelda Bates
Jul 9, 2016·Stem Cells Translational Medicine·Kiran BattaValerie Kouskoff
Jul 10, 2016·Tissue Engineering. Part C, Methods·Jaichandran SivalingamSteve Kah-Weng Oh
Jul 13, 2016·Stem Cells Translational Medicine·Emmanuel N OlivierJoanne C Mountford
Jan 1, 2017·Cell Biology and Toxicology·Bárbara Cristina Martins Fernandes PaesVirginia Picanço-Castro
Oct 28, 2017·Methods in Molecular Biology·Anna KovilakathJoyce A Lloyd
Oct 28, 2017·Methods in Molecular Biology·Divya S Vinjamur, Daniel E Bauer
Mar 22, 2018·International Journal of Molecular Sciences·Hirofumi NoguchiYoshiki Nakashima
May 17, 2018·Molecular Therapy. Methods & Clinical Development·Naoya UchidaJohn F Tisdale
Jan 9, 2019·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Evangelia-Eleni ChristakiGeorge Vassilopoulos
Feb 23, 2019·Nature Communications·Martha Lopez-YrigoyenLesley M Forrester

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Citations

Oct 25, 2020·Transfusion·Kshitij SrivastavaWilly A Flegel
Nov 18, 2020·Stem Cell Research & Therapy·Mohsen EbrahimiMaryam Farzaneh
May 6, 2021·Cell Reports Medicine·Kristina G Maxwell, Jeffrey R Millman
Nov 2, 2021·Stem Cells Translational Medicine·Xiaoyan XieXuetao Pei

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Methods Mentioned

BETA
flow cytometry
transfection
flow

Software Mentioned

Ocular Image Acquisition Software ( OCULAR
FlowJo

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