Human induced pluripotent stem cells differentiate into insulin-producing cells able to engraft in vivo

Acta Diabetologica
Silvia PellegriniValeria Sordi

Abstract

New sources of insulin-secreting cells are strongly required for the cure of diabetes. Recent successes in differentiating embryonic stem cells, in combination with the discovery that it is possible to derive human induced pluripotent stem cells (iPSCs) from somatic cells, have raised the possibility that patient-specific beta cells might be derived from patients through cell reprogramming and differentiation. In this study, we aimed to obtain insulin-producing cells from human iPSCs and test their ability to secrete insulin in vivo. Human iPSCs, derived from both fetal and adult fibroblasts, were differentiated in vitro into pancreas-committed cells and then transplanted into immunodeficient mice at two different stages of differentiation (posterior foregut and endocrine cells). IPSCs were shown to differentiate in insulin-producing cells in vitro, following the stages of pancreatic organogenesis. At the end of the differentiation, the production of INSULIN mRNA was highly increased and 5 ± 2.9 % of the cell population became insulin-positive. Terminally differentiated cells also produced C-peptide in vitro in both basal and stimulated conditions. In vivo, mice transplanted with pancreatic cells secreted human C-peptide in res...Continue Reading

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Citations

Aug 5, 2015·Journal of Clinical Medicine·Meilyn HewMichaela Lucas
Mar 1, 2016·Acta Diabetologica·Silvia PellegriniLorenzo Piemonti
Sep 2, 2015·Biochemical Pharmacology·Veronica IacovacciPaolo Dario
Dec 17, 2015·Singapore Medical Journal·Alper YenigunAysenur Meric
Nov 3, 2016·Cell Biology International·Maryam KavianiIsmail Al-Abdullah
Feb 26, 2016·International Journal of Molecular Sciences·Mohammed Kawser HossainSsang-Goo Cho
Jul 16, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ankur Garg, Xin Zhang
Jun 28, 2018·Tissue Engineering. Part B, Reviews·Caden DuffyAlonzo D Cook
Jan 1, 2017·Acta Diabetologica·Guido SebastianiFrancesco Dotta
Jan 9, 2021·The Journal of Clinical Endocrinology and Metabolism·Silvia PellegriniLorenzo Piemonti
May 25, 2021·Acta Diabetologica·Lorenzo Piemonti

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