Human iNPC therapy leads to improvement in functional neurologic outcomes in a pig ischemic stroke model

Brain and Behavior
Vivian W LauFranklin D West

Abstract

Stroke is the leading cause of disability in the United States but current therapies are limited with no regenerative potential. Previous translational failures have highlighted the need for large animal models of ischemic stroke and for improved assessments of functional outcomes. The aims of this study were first, to create a post-stroke functional outcome assessment scale in a porcine model of middle cerebral artery occlusion (MCAO) and second, to use this scale to determine the effect of human-induced-pluripotent-cell-derived neural progenitor cells (iNPCs) on functional outcome in this large animal stroke model. Eight 6-month-old Landrace mix pigs underwent permanent MCAO. Five days following MCAO, pigs received intraparenchymal injections of either iNPCs or PBS. A post-stroke assessment scale was developed to measure functional outcome. Evaluations were performed at least 1-3 days prior to MCAO and repeated 1 day, 3 days, and 5 days post-stroke as well as 1 day, 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks, 9 weeks, and 12 weeks post-injection. Comparisons of scores between animals receiving iNPCs or PBS only were compared using a two-way ANOVA and a Tukey's post-hoc t test. The developed scale was able to consistently deter...Continue Reading

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Citations

Dec 5, 2019·International Journal of Molecular Sciences·Roxana SurugiuAurel Popa-Wagner
Sep 12, 2020·International Journal of Molecular Sciences·Marc Melià-SorollaTeresa Gasull
Sep 18, 2020·International Journal of Molecular Sciences·Satoshi SudaKazumi Kimura
Jun 29, 2021·Animal Models and Experimental Medicine·Sunil K NarayanMurugesan Arumugam
Jun 18, 2021·Frontiers in Neuroscience·Ranran DuanYanjie Jia
Oct 30, 2021·Frontiers in Genome Editing·Henriette Reventlow FrederiksenKristine K Freude

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