PMID: 9445392Mar 7, 1998Paper

Human insulin production from a novel mini-proinsulin which has high receptor-binding activity

The Biochemical Journal
S G ChangH C Shin

Abstract

To increase the folding efficiency of the insulin precursor and the production yield of insulin, we have designed a mini-proinsulin (M2PI) having the central C-peptide region replaced with a sequence forming a reverse turn. The mini-proinsulin was fused at the N-terminus to a 21-residue fusion partner containing a His10 tag for affinity purification. The gene for the fusion protein was inserted downstream of the T7 promoter of the expression plasmid pET-3a, and the fusion proteins were produced as inclusion bodies in the Escherichia coli cytoplasm at levels up to 25% of the total cell protein. The protein was sulphonated, cleaved by CNBr and the M2PI mini-proinsulin was purified using ion-exchange chromatography. The refolding yield of M2PI was 20-40% better than that of proinsulin studied at the same molar concentrations, indicating that the short turn-forming sequence is more effective in the refolding process than the much longer C-peptide. Native human insulin was successfully generated by subsequent enzymic conversion of mini-proinsulin. The mini-proinsulin exhibited high receptor-binding activity, about 50% as potent as insulin, suggesting that this single-chained mini-proinsulin may provide a foundation in understanding ...Continue Reading

Citations

Dec 18, 2007·Preparative Biochemistry & Biotechnology·El-Rashdy M RedwanEhab A Serour
Jul 20, 2010·Journal of Peptide Science : an Official Publication of the European Peptide Society·Asser S AndersenThomas Hoeg-Jensen
Oct 23, 2019·Applied Microbiology and Biotechnology·Ruifeng MaoYefu Wang
Mar 12, 2008·The Journal of Biological Chemistry·Qing-xin HuaMichael A Weiss
Feb 26, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Fa LiuJohn P Mayer
Nov 21, 2019·Protein and Peptide Letters·B N Nandini B Anthembio ComGanesh Sambasivam
Nov 28, 2008·Chembiochem : a European Journal of Chemical Biology·A Pernille ToftengThomas Hoeg-Jensen
Mar 21, 2020·Chemistry : a European Journal·Mads ØstergaardKnud J Jensen
Aug 5, 2017·Accounts of Chemical Research·Fa LiuRichard DiMarchi
Jul 26, 2019·ACS Chemical Biology·Florence M BrunelRichard D DiMarchi
Aug 5, 2004·Journal of the American Chemical Society·Derek Macmillan, Lubna Arham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.