Human interleukin 1 beta: corticotropin releasing factor and ACTH release and gene expression in the male rat: in vivo and in vitro studies

Journal of Neuroscience Research
S Mélik ParsadaniantzB Kerdelhué

Abstract

Numerous studies have shown that interleukin 1 (IL1), a cytokine secreted by macrophages, is capable of stimulating the hypothalamo-pituitary-adrenal (HPA) axis. Nevertheless, the sites involved in IL1 stimulation of the HPA axis remain, to date, subjects of controversy. In the present study, using in vivo and in vitro approaches, we tried to characterize the route by which IL1 acts on the HPA axis. In vivo, after an i.p. injection of human IL1 beta (1 microgram/rat), we measured plasma ACTH concentration, anterior pituitary (AP) ACTH content, hypothalamic (HT) corticotropin releasing factor (CRF) content, and also AP pro-opiomelanocortin (POMC) and HT CRF gene expression. ACTH and CRF were measured by specific radioimmunoassays (RIAs), and solution hybridization nuclease protection assay was used for quantification of nuclear POMC precursor RNA and nuclear and cytoplasmic POMC and CRF mRNA. Human IL1 beta provoked an increase in ACTH plasma concentration, a decrease in AP ACTH content, and a prolonged increase in AP POMC primary transcript levels (around 100%). A significant increase in AP POMC primary transcript content was evident 30 min after injection of hIL1 beta, while cytoplasmic POMC mRNA levels were increased in the A...Continue Reading

References

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Citations

Oct 23, 1997·Journal of Neuroimmunology·P D Lyons, J E Blalock
Jul 11, 1998·Alcoholism, Clinical and Experimental Research·D D RasmussenC W Wilkinson
Dec 20, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Anna FeldreichAnnika Rosén
Jul 6, 2016·Frontiers in Endocrinology·Djillali Annane
Nov 26, 2005·Brain, Behavior, and Immunity·Nicholas Cohen
Aug 10, 2006·American Journal of Physiology. Endocrinology and Metabolism·Annabelle Reaux-Le GoazigoCatherine Llorens-Cortes
Aug 14, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Federica BarbieriTullio Florio

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