Human Interleukin-32γ Plays a Protective Role in an Experimental Model of Visceral Leishmaniasis in Mice

Infection and Immunity
Rodrigo Saar GomesFátima Ribeiro-Dias

Abstract

Visceral leishmaniasis (VL) is a chronic parasitic disease caused by Leishmania infantum in the Americas. During VL, several proinflammatory cytokines are produced in spleen, liver, and bone marrow. However, the role of interleukin-32 (IL-32) has not been explored in this disease. IL-32 can induce production of proinflammatory cytokines in innate immune cells and polarize the adaptive immune response. Herein, we discovered that L. infantum antigens induced expression of mRNA mainly for the IL-32γ isoform but also induced low levels of the IL-32β transcript in human peripheral blood mononuclear cells. Furthermore, infection of human IL-32γ transgenic mice (IL-32γTg mice) with L. infantum promastigote forms increased IL-32γ expression in the spleen and liver. Interestingly, IL-32γTg mice harbored less parasitism in the spleen and liver than wild-type (WT) mice. In addition, IL-32γTg mice showed increased granuloma formation in the liver compared to WT mice. The protection against VL was associated with increased production of nitric oxide (NO), interferon gamma (IFN-γ), IL-17A, and tumor necrosis factor alpha by splenic cells restimulated ex vivo with L. infantum antigens. In parallel, there was an increase in the number of Th1 a...Continue Reading

References

Dec 1, 1985·The Journal of Clinical Investigation·E M CarvalhoW D Johnson
Dec 1, 1993·Journal of Cellular Biochemistry·S L Reiner, R M Locksley
Jul 1, 1993·The Journal of Clinical Investigation·H W GhalibS G Reed
Apr 1, 1993·Experimental Cell Research·C J SympsonT E Geoghegan
Aug 26, 1998·FEMS Immunology and Medical Microbiology·S HonoréF Derouin
Feb 12, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J LehmannM L Lohmann-Matthes
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K R GanttM E Wilson
Aug 9, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·P Desjeux
Nov 13, 2001·International Journal of Experimental Pathology·H W Murray
Jul 27, 2004·Memórias do Instituto Oswaldo Cruz·Sebastião Martins de Souza-NetoLuís Carlos Crocco Afonso
Sep 14, 2004·Immunological Reviews·Paul M KayeChristian R Engwerda
Aug 30, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Asrat HailuPiet A Kager
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Leo A B JoostenWim B van den Berg
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah C HigginsKingston H G Mills
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcel F NoldCharles A Dinarello
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susanna Lopez KostkaEsther von Stebut
Jul 22, 2009·The Journal of Clinical Investigation·Maira G R PittaAlain Dessein
Jul 25, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gopal MurugaiyanHoward L Weiner
Nov 7, 2009·Revista Do Instituto De Medicina Tropical De São Paulo·Hiro Goto, Maria das Graças Prianti
Mar 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiyuan BaiEdward D Chan
May 6, 2010·The Journal of Infectious Diseases·Marije OostingLeo A B Joosten
Oct 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei LiYing Zhu
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jida ChoiSoohyun Kim
Jan 14, 2011·Parasite Immunology·R NovoaE M Carvalho
Jan 1, 2008·CSH Protocols·Joachim Weischenfeldt, Bo Porse
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bas HeinhuisLeo A B Joosten
May 10, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mi Young JungTae Sung Kim
Jul 13, 2011·Nature Reviews. Microbiology·Paul Kaye, Phillip Scott
Oct 12, 2011·Parasites & Vectors·Clarisa B Palatnik-de-Sousa, Michael J Day
Jun 13, 2012·The Journal of Infection·Qihuan XuGang Li

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Citations

Sep 2, 2020·Journal of Leukocyte Biology·Kristin Roseth AassTherese Standal
Nov 1, 2020·Cytokine·Renuka BhorKalpana Pai
Jul 15, 2021·Microbial Pathogenesis·Muriel Vilela Teodoro SilvaFátima Ribeiro-Dias

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