Human intestinal epithelial cell line SK-CO15 is a new model system to study Na(+)/H(+) exchanger 3.

American Journal of Physiology. Gastrointestinal and Liver Physiology
Byong Kwon YooC Chris Yun

Abstract

The Caco-2 cell line represents absorptive polarized intestinal epithelial cells that express multiple forms of Na(+)/H(+) exchanger (NHE) in their plasma membranes. Caco-2 cells express the major apical NHE isoform NHE3, but low NHE3 expression together with inefficient transfection often hamper intended studies. In this study, we examined whether SK-CO15 cells could be used to study NHE3 regulation. SK-CO15 cells grown on Transwell inserts developed polarized epithelial cells with microvilli. The transfection efficiency of SK-CO15 cells was markedly higher compared with Caco-2 cells, an advantage in gene transfer and knockout. SK-CO15 cells expressed NHE1, NHE2, and NHE3. NHE3 expression was significantly greater in these cells than Caco-2, and NHE3 comprised more than half of total NHE activity. Apical expression of NHE3 in SK-CO15 cells was confirmed by confocal immunofluorescence and surface biotinylation. NHE regulatory factors NHERF1 and NHERF2, which are important for regulation of NHE3 activity, were expressed in these cells. Stimulatory response of NHE3 in SK-CO15 cells was assessed by dexamethasone and lysophosphatidic acid (LPA). Treatment with dexamethasone for 24-48 h increased NHE3 expression and activity. Simila...Continue Reading

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Citations

Oct 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Varsha SinghPradeep K Dudeja
Aug 11, 2015·The Journal of Clinical Investigation·Peijian HeC Chris Yun
Apr 9, 2015·Molecular Biology of the Cell·Varsha SinghMark Donowitz
Jun 10, 2016·American Journal of Physiology. Renal Physiology·Peijian HeC Chris Yun
May 25, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kayte A JenkinC Chris Yun
Nov 24, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anoop KumarSeema Saksena
Sep 5, 2019·American Journal of Physiology. Cell Physiology·Anoop KumarPradeep K Dudeja
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Oct 24, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yiran Han, C Chris Yun
May 12, 2021·Cellular and Molecular Gastroenterology and Hepatology·Mo WangC Chris Yun

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