Human intestinal epithelial cells swell and demonstrate actin rearrangement in response to the metalloprotease toxin of Bacteroides fragilis.

Infection and Immunity
S S KoshyCynthia L Sears

Abstract

Enterotoxigenic Bacteroides fragilis (ETBF) cells produce a 20-kDa heat-labile metalloprotease toxin which is potentially important in the pathogenesis of diarrhea associated with this infection. Previous studies indicate that subconfluent HT29/C1 cells treated with the B. fragilis toxin (BFT) develop morphologic changes with dissolution of tight clusters and apparent swelling. Such alterations suggest toxin-stimulated reorganization of the cellular cytoskeleton. The purpose of the current study was to evaluate the effect of BFT on actin microfilaments (F-actin) and cell volume. As assessed by fluorescent phallicidin staining which detects F-actin, BFT treatment of HT29/C1 cells resulted in redistribution of F-actin with loss of stress fibers, a floccular staining pattern, and cellular membrane blebbing without quantitative changes in F-actin fluorescence intensity. The F-actin redistribution was time and concentration dependent. In contrast to the cell shrinkage observed in response to the F-actin-depolymerizing agents cytochalasin D and Clostridium difficile toxin A, BFT stimulated an increase in HT29/C1 cell volume of 10 to 25% (compared with control cells) over a 24-h time course. Only 10 to 30 ng of BFT per ml was necessar...Continue Reading

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Citations

Aug 16, 2002·Molecular Microbiology·Augusto A FrancoCynthia L Sears
May 9, 2008·Current Infectious Disease Reports·John Holton
Apr 16, 2009·Clinical Microbiology Reviews·Cynthia L Sears
Jan 29, 2005·Inflammatory Bowel Diseases·Carlos MedinaJuan Ramón Malagelada
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S WuC L Sears
Sep 22, 2006·Infection and Immunity·Cynthia L SearsAugusto A Franco
Feb 26, 2000·Infection and Immunity·S Zhang, C W Maddox
Jul 13, 2006·Current Microbiology·Viviane NakanoMario J Avila-Campos
Apr 16, 2002·Infection and Immunity·Shaoguang WuCynthia L Sears
Jan 13, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christa N GrantTracy C Grikscheit
Mar 16, 2017·IUBMB Life·Yuta HatoriReiko Akagi
Dec 21, 2019·Journal of Molecular Biology·Ezequiel Valguarnera, Juliane Bubeck Wardenburg
Apr 4, 2021·International Journal of Molecular Sciences·Tracy ParadisFabienne Bon
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·C L Sears
Oct 8, 1997·Journal of Clinical Microbiology·A PantostiF D'Ambrosio
Mar 1, 1997·Infection and Immunity·A A FrancoC L Sears

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