PMID: 6168659Aug 1, 1981Paper

Human intestinal mucosal mast cells: evaluation of fixation and staining techniques

Journal of Clinical Pathology
S StrobelA Ferguson

Abstract

The staining properties of tissue mast cells are influenced by the method of fixation. Differences in fixation and staining techniques may explain the contradictory results in the published reports on the number of human mucosal mast cells (MMC) in the gastrointestinal mucosa in health and disease. We have examined the influence of fixatives on the staining properties of human MMC in operative biopsy specimens of human jejunum. Specimens were divided into pieces, each of which was fixed in one of the following fixatives: Carnoy's, basic lead acetate (BLA), Baker's, Bouin's, isotonic formol-acetic-acid (IFAA), 10% neutral buffered formalin, formol sublimate, and formol saline. Thereafter, tissues were paraffin-embedded and 5 micron sections were cut and stained with either astra-blue/safranin pH 0.3, or toluidine blue pH 0.5. Counts of the number of MMC/mm2 were obtained for each fixation method. The results show a critical influence of the fixative on the number of mast cells identified after staining. For example with astra-blue/safranin the mean MMC/mm2 count was 40 in formol-saline-fixed specimens, and 268 in Carnoy's-fixed specimens. In biopsies fixed with formalin-based fixatives, mast cells were more readily stained with ...Continue Reading

References

Sep 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Tas, R G Berndsen
Sep 14, 1979·JAMA : the Journal of the American Medical Association·S Kocoshis, J D Gryboski
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R G WoodburyD Lagunoff
Jul 1, 1979·Gut·K SjölundF Sundler
Jan 1, 1979·Journal of Clinical Pathology·N J Dodd, M G Bond
Feb 1, 1979·Journal of Cutaneous Pathology·G D MonheitM Conrad
Jun 1, 1979·Medical Hypotheses·B A Young, G R Dickson
Jan 1, 1966·Acta Pathologica Et Microbiologica Scandinavica·L Enerbäck
Jan 1, 1980·Scandinavian Journal of Gastroenterology·B H SelbekkL E Hanssen
Sep 1, 1980·Archives of Disease in Childhood·L DollbergS Freier
Jan 1, 1960·Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie·G BLOOM, J W KELLY

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Citations

Jan 1, 1996·Lasers in Surgery and Medicine·S O el Sayed, M Dyson
Dec 1, 1989·The American Journal of Anatomy·E L StockS I Roth
Jan 11, 1991·Arthritis and Rheumatism·C B Lindsley, P B Miner
Jul 1, 1993·The Histochemical Journal·L R XuG A Hall
Jun 1, 1992·Cell and Tissue Research·H Chiarini-Garcia, C R Machado
Sep 1, 1993·Cell and Tissue Research·Y Ikeda, S Yamashina
Jun 1, 1994·Cell and Tissue Research·J S MarshallJ Bienenstock
Nov 1, 1984·Biulleten' eksperimental'noĭ biologii i meditsiny·I V ZverkovL I Aruin
Jul 1, 1984·Journal of Clinical Immunology·K E Barrett, D D Metcalfe
Sep 1, 1988·The Histochemical Journal·U PipkornL Enerbäck
Apr 1, 1985·The Histochemical Journal·D Hopwood
Apr 11, 1992·Digestive Diseases and Sciences·T KingP B Miner
Nov 1, 1990·Digestive Diseases and Sciences·S E MatterP B Miner
Nov 1, 1990·Digestive Diseases and Sciences·P GoldsmithD A Robertson
Mar 1, 1988·The Histochemical Journal·C J KirkpatrickR W Stoddart
Apr 1, 1988·Agents and Actions·F L Pearce
Apr 1, 1989·Agents and Actions·M K ChurchR C Benyon
Jan 1, 1989·Immunologic Research·S S Craig, L B Schwartz
Nov 15, 2006·Digestive Diseases and Sciences·Barbara FranceschiniFabio Grizzi
Sep 1, 1990·The British Veterinary Journal·W ChenP Slack
Apr 1, 1989·International Journal for Parasitology·T L Rothwell
Jan 1, 1988·Leukemia Research·J H ButterfieldG J Gleich
Jun 11, 1992·Regulatory Peptides·M I Ledesma de PaoloL B Fernandez

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