Human intrahepatic biliary epithelial cells function in innate immunity by producing IL-6 and IL-8 via the TLR4-NF-kappaB and -MAPK signaling pathways

Liver International : Official Journal of the International Association for the Study of the Liver
Terufumi YokoyamaHiromi Ishibashi

Abstract

Human intrahepatic biliary epithelial cells (HIBECs) may play active roles in both the innate and adaptive immune responses. Little is known, however, about the role of toll-like receptors (TLRs) on HIBECs in inflammatory cholangiopathies. The expression of TLR1-9 and the biological responses to their ligands, lipopolysaccharide (LPS) or lipoteichoic acid (LTA), were studied in cultured HIBECs by reverse transcription-polymerase chain reaction, immunoblotting, and enzyme-linked immunosorbent assay. HIBECs constitutively expressed transcripts encoding TLR1-6 and 9, as well as myeloid differentiation factor 88 (MyD88), MD2, and CD14. Stimulation of HIBECs with LPS resulted in translocation of NF-kappaB subunits from the cytoplasmic to the nuclear fraction, followed by increased secretion of a variety of chemokines/cytokines, including interleukin-8 (IL-8), monocyte chemotactic protein-1 (MCP-1), and IL-6. Treatment with BAY11-7082 efficiently inhibited the LPS-induced transcription and secretion of these chemokines/cytokines. In HIBECs, the mitogen-activated protein kinases (MAPKs) were also activated by LPS stimulation. These results indicated that LPS activates HIBECs via a TLR4-MyD88-dependent pathway. Stimulation of HIBECs wi...Continue Reading

References

Apr 10, 1999·The Journal of Biological Chemistry·J C ChowF Gusovsky
Feb 19, 2000·Current Opinion in Immunology·K V Anderson
Sep 22, 2000·The Biochemical Journal·S P DaviesP Cohen
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Q JiangH R Petty
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J D SchillingS J Hultgren
Mar 23, 2002·Biochimica Et Biophysica Acta·Tsuneyasu Kaisho, Shizuo Akira
Jun 11, 2002·Gastroenterology·M HausmannG Rogler
Aug 9, 2002·The New England Journal of Medicine·Daniel K Podolsky
Jan 22, 2003·The Biochemical Journal·Jenny BainPhilip Cohen
Aug 16, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yasuji TeradaYasuhiko Shimizu
Nov 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kenichi HaradaYasuni Nakanuma
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kristine PhillipsPaul Anderson
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichi TabetaBruce Beutler
Jun 12, 2004·American Journal of Respiratory Cell and Molecular Biology·Quan ShaRobert P Schleimer
Jun 25, 2004·American Journal of Reproductive Immunology : AJRI·Steven L YoungMichael L Misfeldt
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Jul 22, 2004·Journal of Neuroimmunology·Matam Vijay KumarBarbara Detrick
Dec 25, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takashi KamihiraMine Harada
Apr 28, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yasushi TakiiHiromi Ishibashi
Jul 12, 2005·Journal of Autoimmunity·Ai-Ping WangHiromi Ishibashi

❮ Previous
Next ❯

Citations

Jun 13, 2012·Clinical Reviews in Allergy & Immunology·Kazuhito KawataChristopher L Bowlus
Oct 1, 2013·Journal of Autoimmunity·Jorge Henao-MejiaRichard A Flavell
May 28, 2008·Immunology and Cell Biology·Xian-Ming ChenNicholas F LaRusso
Feb 24, 2011·Seminars in Liver Disease·Luca Fabris, Mario Strazzabosco
Jul 16, 2011·The Journal of Biological Chemistry·Steven P O'HaraNicholas F LaRusso
Jan 31, 2008·Postgraduate Medical Journal·D E J Jones
Sep 11, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gaurav SyalJonathan A Dranoff
May 14, 2009·The Journal of Clinical Investigation·Zhaoyu LiKlaus H Kaestner
Oct 23, 2010·Fibrogenesis & Tissue Repair·Jinsheng Guo, Scott L Friedman
Nov 16, 2013·BioMed Research International·James H TabibianKeith D Lindor
May 27, 2009·Seminars in Immunopathology·Hiromi IshibashiShinji Shimoda
Jun 18, 2009·Seminars in Immunopathology·Ya-Hui ChuangM Eric Gershwin
Oct 1, 2008·Journal of Cancer Research and Clinical Oncology·Yigang QianShusen Zheng
Jan 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shinji ShimodaM Eric Gershwin
Oct 13, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kenichi Harada, Yasuni Nakanuma
Oct 13, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Atsumasa KomoriHiromi Ishibashi
Apr 25, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Kenichi HaradaYasuni Nakanuma
May 15, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Kumiko IsseYasuni Nakanuma
Nov 19, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Tobias MuellerDaniel K Podolsky
Sep 14, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yasuhiro Miyake, Kazuhide Yamamoto
Nov 25, 2011·Proteomics·Mustafa A BarbhuiyaAkhilesh Pandey
Sep 11, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Yoshihiro AibaMinoru Nakamura
Nov 13, 2015·Liver International : Official Journal of the International Association for the Study of the Liver·James H TabibianSteven P O'Hara
Jan 23, 2015·Journal of Gastroenterology and Hepatology·Rui Dong, Shan Zheng
Nov 25, 2011·Gastroenterology Research and Practice·A G Miranda-DíazL M Román-Pintos
Aug 8, 2015·BioMed Research International·Maria Grazia ClementeKathleen B Schwarz
Oct 14, 2011·International Journal of Hepatology·Kenichi Harada, Yasuni Nakanuma
Oct 16, 2014·Autoimmunity Reviews·Hong-Di MaZhe-Xiong Lian
Feb 6, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A J DemetrisD B Stolz
Feb 13, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·Serge J ZweersFrank G Schaap
Apr 9, 2011·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·X ZhangX Ling
Nov 20, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·Takeji UmemuraMasao Ota
Jul 30, 2017·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Claudio PintoMarco Marzioni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.