Human iPS Cell-Derived Patient Tissues and 3D Cell Culture Part 2: Spheroids, Organoids, and Disease Modeling

SLAS Technology
Richard M Eglen, Terry Reisine

Abstract

Human induced pluripotent stem cells (HiPSCs) provide several advantages for drug discovery, but principally they provide a source of clinically relevant tissue. Furthermore, the use of HiPSCs cultured in three-dimensional (3D) systems, as opposed to traditional two-dimensional (2D) culture approaches, better represents the complex tissue architecture in vivo. The use of HiPSCs in 3D spheroid and organoid culture is now growing, but particularly when using myocardial, intestinal enteric nervous system, and retinal cell lines. However, organoid cell culture is perhaps making the most notable impact in research and drug discovery, in which 3D neuronal cell cultures allow direct modeling of cortical cell layering and neuronal circuit activity. Given the specific degeneration seen in discrete neuronal circuitry in Alzheimer's disease (AD) and Parkinson's disease (PD), HiPSC culture systems are proving to be a major advance. In the present review, the second part of a two-part review, we discuss novel methods in which 3D cell culture systems (principally organoids) are now being used to provide insights into disease mechanisms. (The use of HiPSCs in target identification was reviewed in detail in Part 1.).

References

Dec 1, 1978·Gut·J H Scarpello, G E Sladen
Feb 9, 2002·Circulation Research·W-H ZimmermannT Eschenhagen
Mar 12, 2003·The Journal of Comparative Neurology·Martin AnlaufEberhard Weihe
Jun 12, 2004·Journal of Biomolecular Screening·Leoni A Kunz-SchughartReinhard Ebner
Nov 23, 2006·Lancet·Dyonne T HartongThaddeus P Dryja
Feb 3, 2007·Orphanet Journal of Rare Diseases·Christian P Hamel
Apr 15, 2008·Current Opinion in Ophthalmology·Peter Goodwin
May 6, 2008·Seminars in Cancer Biology·Britta Weigelt, Mina J Bissell
May 8, 2010·Circulation Research·Arne HansenThomas Eschenhagen
Jul 20, 2010·Journal of Biomolecular Screening·Fredika M RobertsonSanford H Barsky
May 21, 2011·Circulation Research·Nathaniel L TullochCharles E Murry
Nov 10, 2011·Neurology·P DerkinderenR De Giorgio
Nov 16, 2011·Nature Protocols·Kyle W McCrackenJason R Spence
Jul 12, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L WangY Taché
Jul 13, 2012·Expert Opinion on Biological Therapy·Toni-Marie AchilliJeffrey R Morgan
Oct 18, 2012·Drug Discovery Today·Susan Breslin, Lorraine O'Driscoll
Oct 27, 2012·Basic Research in Cardiology·Marc N HirtThomas Eschenhagen
Dec 3, 2013·Nature Biotechnology·Sarah X L HuangHans-Willem Snoeck
Mar 4, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Andrea StoehrArne Hansen
Jun 11, 2014·Nature Communications·Xiufeng ZhongM Valeria Canto-Soler
Jul 19, 2014·Science·Madeline A Lancaster, Juergen A Knoblich
Mar 10, 2015·Journal of Biotechnology·Oliver Ingo HoffmannBarbara Mayer
Mar 10, 2015·Scientific Reports·Anurag MathurKevin E Healy
Jan 1, 2014·Journal of Clinical Medicine·Fred K ChenDavid A Mackey
Aug 25, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ruchira SinghDavid M Gamm
Sep 24, 2015·Expert Opinion on Drug Discovery·Natalia J MartinezAnton Simeonov
Nov 27, 2015·Methods : a Companion to Methods in Enzymology·Linfeng LiDaniel V LaBarbera
Jan 11, 2016·Cell Stem Cell·Xiaolei YinOren Levy
Jan 23, 2016·Nature Protocols·Jarno DrostHans Clevers
Apr 12, 2016·Pharmacology & Therapeutics·Sritama Nath, Gayathri R Devi

❮ Previous
Next ❯

Citations

Jan 21, 2020·SLAS Technology·Edward Kai-Hua Chow
Jun 10, 2020·Military Medicine·Jordan F BetzJoel D Gaston
Jan 23, 2021·Genes·Marta García-LópezM Esther Gallardo
Aug 28, 2021·International Journal of Molecular Sciences·Mohammad Saied SalehiSareh Pandamooz

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
biosensor
chips
transgenic

Related Concepts

Related Feeds

Brain Organoids in Disease Modeling

Brain organoids are three-dimensional cell culture models derived from human pluripotent stem cells. Since they resemble the embryonic brain, they can be used to help study brain biology, early brain development, and brain diseases. Discover the latest research on brain organoids in disease modeling here.

3D Cellular Models of Brain and Neurodegeneration

Brain organoids are three-dimensional in vitro cellular models of the brain that can recapitulate many processes such as the neurodevelopment. In addition, these organoids can be combined with other cell types, such as neurons and astrocytes to study their interactions in assembloids. Disease processes can also be modeled by induced pluripotent stem cell-derived organoids and assembloids from patients with neurodegenerative disorders. Discover the latest research on the models here.

Related Papers

International Journal of Molecular Sciences
Beatrice Xuan HoBoon-Seng Soh
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Sharmin AlhaqueHassan Rashidi
Current Topics in Microbiology and Immunology
Anna Pomerenke
© 2022 Meta ULC. All rights reserved