Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold.

Biomaterials
Soon Seng NgS Tamir Rashid

Abstract

Generation of human organoids from induced pluripotent stem cells (iPSCs) offers exciting possibilities for developmental biology, disease modelling and cell therapy. Significant advances towards those goals have been hampered by dependence on animal derived matrices (e.g. Matrigel), immortalized cell lines and resultant structures that are difficult to control or scale. To address these challenges, we aimed to develop a fully defined liver organoid platform using inverted colloid crystal (ICC) whose 3-dimensional mechanical properties could be engineered to recapitulate the extracellular niche sensed by hepatic progenitors during human development. iPSC derived hepatic progenitors (IH) formed organoids most optimally in ICC scaffolds constructed with 140 μm diameter pores coated with type I collagen in a two-step process mimicking liver bud formation. The resultant organoids were closer to adult tissue, compared to 2D and 3D controls, with respect to morphology, gene expression, protein secretion, drug metabolism and viral infection and could integrate, vascularise and function following implantation into livers of immune-deficient mice. Preliminary interrogation of the underpinning mechanisms highlighted the importance of TGF...Continue Reading

Citations

Jan 25, 2020·Advanced Healthcare Materials·Alain da Silva MoraisRui L Reis
Feb 6, 2020·Advanced Healthcare Materials·Jun-Goo Kwak, Jungwoo Lee
Oct 2, 2020·Biological Reviews of the Cambridge Philosophical Society·Ogechi OgokeNatesh Parashurama
May 6, 2019·Bioengineering·Kimia Kamal, Ben Waldau
Feb 8, 2020·Tissue Engineering. Part C, Methods·Ryan CarpenterJungwoo Lee
Dec 6, 2019·Frontiers in Medicine·James L Corbett, Stephen A Duncan
Jul 10, 2019·Advanced Materials·Haitao LiuJianhua Qin
Mar 25, 2020·Tissue Engineering and Regenerative Medicine·Sang Woo LeeGi Seok Jeong
Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Chizhu DingXianghua Yan
Oct 19, 2020·Stem Cell Reviews and Reports·Akram NadiShiva Asadpour
Jan 27, 2021·Stem Cell Research & Therapy·Meixian JinShuqin Zhou
Jan 11, 2021·Development, Growth & Differentiation·Wendy L Thompson, Takanori Takebe
Feb 13, 2021·Viruses·Chui-Wa So, Glenn Randall
Apr 3, 2021·Cell Stem Cell·Qiang HuangXiling Shen
May 12, 2021·Acta Biochimica Et Biophysica Sinica·Ting YaoXiaohua Chen
Jul 1, 2021·Critical Reviews in Biotechnology·Amanda Marchini, Fabrizio Gelain
Aug 14, 2021·Nature Materials·Bauer L LeSavageSarah C Heilshorn
Sep 9, 2019·ACS Biomaterials Science & Engineering·Tarun AgarwalTapas Kumar Maiti
Sep 26, 2021·Advanced Materials·Sang Ah YiKi-Bum Lee
Dec 4, 2021·Frontiers in Bioengineering and Biotechnology·Wen-Ming LiuHe-Xin Yan
Dec 12, 2021·Communications Biology·Mark T KozlowskiHsun Teresa Ku

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Methods Mentioned

BETA
biopsy
Assay
enzyme-linked immunosorbent assay
PCR
RNAseq
HT-Seq
flow cytometry
FACS
RNA-seq

Software Mentioned

LAS X
HT
R Development Core
FlowJo
Ingenuity Pathway ( IPA
DESEq2
element
R
GSEA
KMEANS

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