Jan 12, 2020

Human iPSC-derived microglia: A growing toolset to study the brain's innate immune cells

Glia
Jonathan Hasselmann, Mathew Blurton-Jones

Abstract

Recent advances in the generation of microglia from human induced pluripotent stem cells (iPSCs) have provided exciting new approaches to examine and decipher the biology of microglia. As these techniques continue to evolve to encompass more complex in situ and in vivo paradigms, so too have they begun to yield novel scientific insight into the genetics and function of human microglia. As such, researchers now have access to a toolset comprised of three unique "flavors" of iPSC-derived microglia: in vitro microglia (iMGs), organoid microglia (oMGs), and xenotransplanted microglia (xMGs). The goal of this review is to discuss the variety of research applications that each of these techniques enables and to highlight recent discoveries that these methods have begun to uncover. By presenting the research paradigms in which each model has been successful, as well as the key benefits and limitations of each approach, it is our hope that this review will help interested researchers to incorporate these techniques into their studies, collectively advancing our understanding of human microglia biology.

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Mentioned in this Paper

Research Personnel
Immune Effector Cell
In Vivo
Omg protein, mouse
Brain
Research
Induced Pluripotent Stem Cells
Objective (Goal)
In Situ
Organoids

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