PMID: 6113848May 14, 1981Paper

Human jejunal brush border folate conjugase. Characteristics and inhibition by salicylazosulfapyridine

Biochimica Et Biophysica Acta
A M Reisenauer, C H Halsted

Abstract

Human jejunal brush border folate conjugase (EC 3.4.22.-) was partially purified and characterized. Three drugs known to be associated with clinical folate deficiency were tested for inhibition of the partially purified enzyme. Using jejunal mucosa from obese patients undergoing intestinal bypass surgery, brush border folate conjugase was purified 50-80-fold by centrifugation, Triton X-100 solubilization and DEAE-Sephadex and Sephacryl S-200 chromatography. Using synthetic pteroyldiglutamyl[14C]glutamate as substrate, the enzyme was found to have a pH optimum of 6.5 and an apparent Km of 1.6 micro M. Incubation of the enzyme with synthetic pteroyl[14C]glutamylhexaglutamate resulted in a spectrum of shorter-chain 14C-labeled pteroylglutamates at 60 min. Pteroyl[14C]glutamate was the major product at 120 min, with quantitative recovery of free glutamate in the incubation medium. Salicylazosulfapyridine was a competitive inhibitor of the enzyme (Ki = 0.13 mM), while ethanol, diphenylhydantoin and salicylazosulfapyridine metabolites had no effect. These data suggest that brush border folate conjugase is an exopeptidase which progressively hydrolyzes glutamyl units from pteroylpolyglutamate, leaving pteroylmonoglutamate as the folat...Continue Reading

References

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Citations

Jan 1, 1987·Pharmacology & Therapeutics·C H Halsted, C Heise
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J F GregoryJ J Cerda
Dec 17, 1981·The New England Journal of Medicine·C H HalstedT Tamura
Jan 1, 1985·Scandinavian Journal of Rheumatology·K A Grindulis, B McConkey
Jan 11, 1991·Journal of Toxicology. Clinical Toxicology·A MinochaJ E Mayle
Dec 1, 1989·Gut·R Holmes, R W Lobley
Jul 21, 2005·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·G Biasco, M C Di Marco
Dec 1, 1989·Journal of the American College of Nutrition·C H Halsted
Aug 12, 2014·World Journal of Gastroenterology : WJG·Eun Ran Kim, Dong Kyung Chang
Aug 1, 1996·Scandinavian Journal of Clinical and Laboratory Investigation·M Krogh JensenL Svendsen
Jul 25, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Erasmus Schneider, Thomas J Ryan

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