Human keratinocyte cultures (HaCaT) can be infected by DENV, triggering innate immune responses that include IFNλ and LL37.

Immunobiology
Moisés López-GonzálezLeticia Cedillo-Barrón

Abstract

The skin is the first anatomical region that dengue virus (DENV) encounters during the natural infection. Although the role of some skin resident cells like dendritic cells and fibroblasts has been demonstrated to be crucial to elucidate the role of resident cells and molecules participating during the early events of the innate immune response, the participation of keratinocytes during DENV infection has not been fully elucidated. In this paper we aimed to evaluate the use of the HaCaT cell line as a model to study the immune responses of skin keratinocytes to DENV infection. We demonstrated productive DENV-2 infection of HaCaT cells and their capability to establish an antiviral response through production of type I and type III interferons (IFN-β and IFN-λ). The production of these cytokines by HaCaT cells correlated with upregulation of IFN-inducible transmembrane protein-3 (IFITM3) and viperin in bystander, uninfected cells. We also observed an increase in secretion of IL-6 and IL-8. Skin keratinocytes are known to secrete antimicrobial peptides (AMPs) during viral infections. In our model, DENV-2 infected HaCaT cells upregulate the production of cytoplasmic LL-37. We evaluated the dual role of LL-37, HBD2, and HBD3 antivi...Continue Reading

Citations

Aug 4, 2019·Viruses·Aslaa AhmedAarthi Narayanan
Jun 26, 2020·Frontiers in Microbiology·Céline ChessaMagali Garcia
Mar 11, 2020·Virologica Sinica·Lionel Berthoux
Jul 15, 2020·Life Science Alliance·Brittany L UhlornSamuel K Campos
Apr 17, 2019·Transactions of the Royal Society of Tropical Medicine and Hygiene·Alfonso Santiago-TéllezJorge Ismael Castañeda-Sánchez
Aug 7, 2019·Current Medicinal Chemistry·David C Brice, Gill Diamond
Nov 13, 2020·Frontiers in Cellular and Infection Microbiology·Xue YangXiaolin Tang
Nov 17, 2020·Frontiers in Immunology·Alfredo E Montes-GómezLeticia Cedillo-Barrón

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