Human keratinocyte cultures in an in vitro approach for the assessment of surfactant-induced irritation

Toxicology in Vitro : an International Journal Published in Association with BIBRA
R WardR Clothier

Abstract

A specific, mechanistic, in vitro approach for the assessment of human skin irritation potential is outlined for the evaluation of surfactants and the results compared with in vivo human patch test data. The level of free available surfactant monomer and the solubilization of the corn protein zein in vitro were confirmed to be related to surfactant in vivo human skin irritation potential. In vitro cytotoxicity to monolayer keratinocyte cultures could not discriminate between the moderate human skin irritant sodium dodecyl sulfate (SDS) and the mild irritants cocamidopropylbetaine (CA) and Polysorbate 20 (P20). An in vitro stratified differentiated human epidermal equivalent (HEE) exhibited reduced cytotoxicity to the test chemicals, compared with monolayer culture responses, and was able to discriminate between the toxic potential of SDS and CA. Stimulation of interleukin-1alpha release from the A431 human keratinocyte cell line reflected in vivo erythema scores more closely than cytotoxic potential, and coincided with nitric oxide production by macrophages upon exposure to A431-conditioned medium. Combination of these mechanistic assays has allowed a profile of likely in vivo human responses to be approximated. Additional know...Continue Reading

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Citations

Dec 24, 2008·Genes & Nutrition·Giovanna MazzoleniN Steimberg
Oct 22, 2005·Toxicology and Applied Pharmacology·Sari PappinenArto Urtti
Jul 25, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J K LeeP Y Kim
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R D Combes
Jan 1, 2014·Advanced Drug Delivery Reviews·Adrian Roth, Thomas Singer
May 21, 2008·International Journal of Cosmetic Science·Andreas Natsch, Michael Wasescha
Jul 7, 2017·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Neil F Johnson
Jun 29, 2005·International Journal of Toxicology·UNKNOWN Cosmetic Ingredient Review Expert Panel
Feb 15, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Ersin Kasım Ulusoy, Ömer Faruk Bolattürk
Apr 5, 2005·Alternatives to Laboratory Animals : ATLA·Massimo TettamantiNatascia Gamba
Oct 31, 2002·Alternatives to Laboratory Animals : ATLA·Richard ClothierLinda Noble
Nov 16, 2007·Alternatives to Laboratory Animals : ATLA·Robert D Combes
Oct 18, 2001·Alternatives to Laboratory Animals : ATLA·V Zuang
Jun 1, 2005·Trends in Biotechnology·Nirmala BhogalMichael Balls

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