PMID: 7537774May 1, 1995Paper

Human keratinocytes regulate their expression of B7/BB-1 antigen by a unique, calcium-dependent mechanism

The Journal of Investigative Dermatology
A NasirA A Gaspari

Abstract

Previous studies of normal human keratinocytes have indicated that these cells express BB-1 antigen, an important adherence molecule usually associated with "professional" antigen-presenting cells. We studied freshly isolated epidermal cells and noted that the frequency of BB-1-positive cells in normal human skin varied from 2.6 to 7.4% of total epidermal cells. Two-color flow cytometry confirmed that keratinocytes were the major cell in the epidermis that expressed BB-1, because less than 10% of total epidermal Langerhans cells were positive for BB-1. Northern blot analysis of RNA extracted from normal human epidermis revealed low levels of 1.7-kb B7-1 transcripts, which were independent of the presence of epidermal Langerhans cells, again indicating that such transcripts were derived from keratinocytes. Keratinocytes cultured in medium containing low concentrations of extracellular calcium (0.07 mM) expressed low levels of cell-surface BB-1. However, keratinocytes cultured in medium with higher levels of extracellular calcium (1.5 mM) lost cell-surface expression of BB-1. Similarly, low-calcium keratinocytes expressed the 1.7- and 2.9-kb B7-1 transcripts, whereas high-calcium keratinocytes expressed only the 1.7-kb transcript...Continue Reading

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Citations

Apr 16, 1998·The British Journal of Dermatology·M C LittleS Mac Neil
Jul 10, 1999·International Journal of Cancer. Journal International Du Cancer·G R ThomasC Van Waes
Sep 15, 2017·Journal of Oral Science·Alison MarshallStephen Porter

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