Human L-3-phosphoserine phosphatase: sequence, expression and evidence for a phosphoenzyme intermediate

FEBS Letters
J F ColletE Van Schaftingen

Abstract

We report the sequence of the cDNA encoding human L-3-phosphoserine phosphatase. The encoded polypeptide contains 225 residues and shows 30% sequence identity with the Escherichia coli enzyme. The human protein was expressed in a bacterial expression system and purified. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2(+)-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. In addition we found that the enzyme was phosphorylated upon incubation with L-[32P]phosphoserine, which indicates that the reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediate. The sensitivity of the phosphoryl-enzyme to alkali and to hydroxylamine suggests that an aspartyl- or a glutamyl-phosphate was formed. The nucleotide sequence of the cDNA described in this article has been deposited in the EMBL data base under accession number Y10275.

References

Jun 5, 1975·Analytical Biochemistry·J J Witt, R Roskoski
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 11, 1985·Nucleic Acids Research·A F Neuwald, G V Stauffer
Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·M HérinT Boon
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jul 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·L E HokinA Yoda
Jan 1, 1974·Methods in Enzymology·J D Corbin, E M Reimann
Oct 14, 1970·Biochimica Et Biophysica Acta·M IgarashiN Tsuyama
Oct 1, 1970·Archives of Biochemistry and Biophysics·Z B Rose
Jun 1, 1996·Archives of Disease in Childhood·J JaekenE Van Schaftingen
Apr 1, 1958·Biochimica Et Biophysica Acta·L F BORKENHAGEN, E P KENNEDY
Apr 1, 1958·Biochimica Et Biophysica Acta·F C NEUHAUS, W L BYRNE

❮ Previous
Next ❯

Citations

Jul 31, 1998·Protein Science : a Publication of the Protein Society·M C ThallerG M Rossolini
Jan 16, 1998·European Journal of Pharmacology·J E HawkinsonP L Wood
Oct 3, 1998·Trends in Biochemical Sciences·J F ColletV Stroobant
Sep 3, 2010·Quarterly Reviews of Biophysics·Jesper V MøllerPoul Nissen
Mar 10, 2001·Biochemical and Biophysical Research Communications·E StrunckG Vollmer
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·H ChoD Yan
Mar 25, 2010·Animal Genetics·B ZhouJ Y Wang
Mar 10, 2001·Neuron·D H GeschwindH I Kornblum
Dec 16, 2003·European Journal of Human Genetics : EJHG·Maria Veiga-da-CunhaEmile Van Schaftingen
May 13, 2004·Journal of Neuroscience Research·Motohiro ShimizuYasuo Suzuki
Oct 16, 1999·The Journal of Biological Chemistry·I S RidderB W Dijkstra
Jul 4, 2001·The Journal of Biological Chemistry·S AllegriniM G Tozzi
Jul 1, 2020·Cellular and Molecular Life Sciences : CMLS·Giulia MurtasLoredano Pollegioni
Jun 11, 1998·The Journal of Biological Chemistry·J F ColletE Van Schaftingen
Sep 4, 2015·Acta Crystallographica. Section D, Biological Crystallography·Jooyoung ParkNiraj H Tolia
Nov 3, 2017·Amino Acids·M Adeva-AndanyA Domínguez-Montero
Sep 6, 2002·The Journal of Biological Chemistry·Hye-Yeon KimKwang Yeon Hwang
Nov 24, 1999·The Journal of Biological Chemistry·J F ColletE Van Schaftingen
Jul 6, 2005·Biochemistry·Guofeng ZhangKaren N Allen
Jul 9, 2021·Scientific Reports·Tamara DrljacaNevena Veljkovic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.