Human Labor Pain Is Influenced by the Voltage-Gated Potassium Channel KV6.4 Subunit.

Cell Reports
M. C. LeeC Geoffrey Woods

Abstract

By studying healthy women who do not request analgesia during their first delivery, we investigate genetic effects on labor pain. Such women have normal sensory and psychometric test results, except for significantly higher cuff pressure pain. We find an excess of heterozygotes carrying the rare allele of SNP rs140124801 in KCNG4. The rare variant KV6.4-Met419 has a dominant-negative effect and cannot modulate the voltage dependence of KV2.1 inactivation because it fails to traffic to the plasma membrane. In vivo, Kcng4 (KV6.4) expression occurs in 40% of retrograde-labeled mouse uterine sensory neurons, all of which express KV2.1, and over 90% express the nociceptor genes Trpv1 and Scn10a. In neurons overexpressing KV6.4-Met419, the voltage dependence of inactivation for KV2.1 is more depolarized compared with neurons overexpressing KV6.4. Finally, KV6.4-Met419-overexpressing neurons have a higher action potential threshold. We conclude that KV6.4 can influence human labor pain by modulating the excitability of uterine nociceptors.

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Citations

Mar 25, 2021·Journal of Pain Research·Juliane SachauAnthony H Dickenson

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Methods Mentioned

BETA
transfection
X-ray
exome sequencing
Assay
density gradient centrifugation

Software Mentioned

pClamp
Integrated Genome Viewer
R Studio
GraphPad
BLASTP
CANTAB
HEKA
SIFT
Patchmaster
Cn3D

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