Human leukocyte antigen-specific antibodies and gamma-interferon stimulate human microvascular and glomerular endothelial cells to produce complement factor C4.

Transplantation
Rizwan HamerD Zehnder

Abstract

The role of the complement system in antibody-mediated rejection has been investigated in relation to circulating complement interacting with renal microvascular endothelium, resulting in the formation of peritubular capillary C4d. However, the possible importance of local complement synthesis is less clear. The aim of this study was to determine whether human vascular endothelium could produce C4 in response to stimulation in vitro. Human microvascular endothelial cells and glomerular endothelial cells were stimulated with endotoxins, cytokines, and human leukocyte antigen-specific antibodies. Synthesis of complement was investigated using western blotting and indirect immunofluorescence. De novo C4 synthesis was confirmed by using C4 small interfering RNA. Glomerular and microvascular endothelium, both produce C3 and C4 complement protein. Complement synthesis was stimulant-specific-C3 was produced mainly after stimulation with lipopolysaccharide whereas C4 synthesis occurred on treatment with gamma interferon. Culture with human leukocyte antigen-specific antibodies resulted in a significant increase of C4 protein synthesis by both cell lines. We have shown for the first time that human microvascular endothelium can be stimu...Continue Reading

References

Dec 1, 1992·The Journal of Investigative Dermatology·E W AdesT J Lawley
Dec 1, 1991·Clinical and Experimental Immunology·H E FeuchtE Albert
Nov 1, 1989·Biulleten' eksperimental'noĭ biologii i meditsiny·O N ShcheglovitovaT G Orlova
Feb 1, 1985·Journal of Cellular Physiology·S EinhornA Panet
Apr 3, 1969·The New England Journal of Medicine·R Patel, P I Terasaki
Jan 1, 1993·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·J E DawsonE W Ades
Jun 1, 1993·Kidney International·H E FeuchtE Albert
Jul 7, 2000·Brain Research·A KlegerisP L McGeer
Mar 22, 2001·Kidney International·W ZhouS H Sacks
Apr 5, 2001·The New England Journal of Medicine·M J Walport
Apr 12, 2001·The New England Journal of Medicine·M J Walport
Jun 4, 2002·Nature Medicine·Julian R PrattSteven H Sacks
Jun 5, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Paul I Terasaki
Feb 24, 2004·Journal of the American Society of Nephrology : JASN·Simon C SatchellPeter W Mathieson
Jan 28, 2005·Kidney International·Joshua M ThurmanV Michael Holers
Sep 22, 2005·Nature Reviews. Immunology·Robert B Colvin, R Neal Smith
Feb 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Conrad A FarrarSteven H Sacks
Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qi PengWuding Zhou
Mar 28, 2006·The American Journal of Pathology·Tao LinSteven H Sacks
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Lihua BaoRichard J Quigg
Mar 16, 2007·Journal of the American Society of Nephrology : JASN·Robert B Colvin
Sep 4, 2007·Current Opinion in Immunology·Wuding ZhouSteven Sacks
Jul 24, 2010·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Rob HigginsDavid Briggs
Apr 30, 2011·Blood·Christoph Rader

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Citations

Feb 2, 2013·Transplantation·Cinthia B Drachenberg, John C Papadimitriou
Dec 17, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Rob M HigginsDan A Mitchell
Mar 31, 2015·Virchows Archiv : an International Journal of Pathology·Eun Na KimChong Jai Kim
Mar 25, 2015·Trends in Molecular Medicine·Kimberly A ThomasElaine F Reed
Apr 18, 2014·Journal of Immunology Research·William HanfP Toby H Coates
Oct 27, 2016·Immunological Reviews·Lubka T RoumeninaVeronique Fremeaux-Bacchi
Feb 17, 2017·Transplant International : Official Journal of the European Society for Organ Transplantation·Adil H GasimVolker Nickeleit
Oct 28, 2018·Nature Reviews. Nephrology·Ali-Reza BiglarniaBo Nilsson
Feb 12, 2020·Applied Immunohistochemistry & Molecular Morphology : AIMM·Song-Yi ChoiKwang-Sun Suh
Apr 5, 2017·Journal of Immunology Research·Carrie L ButlerElaine F Reed

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