PMID: 9539102Apr 16, 1998Paper

Human leukotriene B4 omega-hydroxylase (CYP4F3) gene: molecular cloning and chromosomal localization

DNA and Cell Biology
Yasushi KikutaM Kusunose

Abstract

Leukotriene B4 (LTB4) omega-hydroxylase catalyzes the conversion of LTB4 into a biologically less active product, 20-hydroxy-LTB4. In a preceding paper (Kikuta et al., 1993), we showed human polymorphonuclear leukocyte (PMN) LTB4 omega-hydroxylase to be a novel form of cytochrome P450, designated CYP4F3, on the basis of its cDNA cloning and expression in yeast cells. Here, we have isolated the gene encoding CYP4F3 and determined its genomic organization and chromosomal localization. The CYP4F3 gene contained 13 exons and spanned approximately 22.2 kb. The cDNA of CYP4F3 contained 5050 nucleotides excluding the poly(A) tail. The translation initiation codon (ATG) was present in exon II. Primer extension and S1 mapping analyses indicated that the transcription initiation site is 49 nucleotides upstream from the 3' end of exon I, and no other initiation sites were detected. A TATA-box-like sequence (TACAT) and 120-b GC-rich sequence were observed just before transcription initiation site. Several putative regulating elements recognized by the GATA family, MZF-1, CACCC binding protein, and C/EBP, were identified in its 5' flanking region. Genomic DNA screening for CYP4F3 and Southern blot analysis suggested the existence of other C...Continue Reading

References

Jul 1, 1992·Archives of Biochemistry and Biophysics·A S MuerhoffE F Johnson
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J KempM G Peterson
Jul 1, 1988·Genes & Development·W H LandschulzS L McKnight
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T EvansG Felsenfeld
Oct 1, 1993·Genes, Chromosomes & Cancer·P AmanF Mitelman
Oct 25, 1993·Nucleic Acids Research·Y MargalitA Fainsod
Jun 1, 1994·DNA and Cell Biology·M V Frolov, V E Alatortsev
Feb 1, 1993·Archives of Biochemistry and Biophysics·C N PalmerE F Johnson
Dec 26, 1995·Biochemical and Biophysical Research Communications·H Kawashima, H W Strobel
Nov 7, 1996·Nature·P R DevchandW Wahli
Jan 1, 1996·Cytogenetics and Cell Genetics·H MohrenweiserK Johnson

❮ Previous
Next ❯

Citations

Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Yasushi KikutaMasamichi Kusunose
Feb 8, 2003·Biochimica Et Biophysica Acta·X CuiH W Strobel
Oct 18, 2002·Gene·Xiaoming Cui, Henry W Strobel
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·J A Hankin, R C Murphy
Feb 13, 2001·Biochemical and Biophysical Research Communications·J BylundE H Oliw
May 9, 2002·Drug Metabolism Reviews·Slobodan Rendic
Nov 6, 2007·Biochimica Et Biophysica Acta·Yasushi KikutaHideo Ohkawa
Jul 9, 2004·Biochimica Et Biophysica Acta·Yasushi KikutaKiyofumi Nakata
Aug 24, 2006·Pharmacology & Therapeutics·Auinash Kalsotra, Henry W Strobel
Jul 16, 1999·Biochemical and Biophysical Research Communications·J BylundE H Oliw
May 18, 2004·Journal of Lipid Research·Valérie Le QuéréJean-Pierre Salaün
Jun 30, 2019·World Journal of Gastroenterology : WJG·Alaattin Sen, Holger Stark
Apr 24, 2003·The Journal of Biological Chemistry·Peter ChristmasRoy J Soberman
Apr 24, 2003·The Journal of Biological Chemistry·Karin A Zemski BerryRobert C Murphy
Jul 20, 2001·The Journal of Biological Chemistry·P ChristmasR J Soberman
Feb 24, 2001·Archives of Biochemistry and Biophysics·Y KikutaM Kusunose
Sep 16, 1999·Archives of Biochemistry and Biophysics·Y KikutaM Kusunose
Jun 3, 2021·International Journal of Molecular Sciences·Yingxia SongYuji Ishii

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.