Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose

Scientific Reports
Minju LeeSeunghun Hong

Abstract

We report a strategy for the human-like smelling of a rose scent utilizing olfactory receptor nanodisc (ND)-based bioelectronic nose devices. In this strategy, a floating electrode (FE)-based carbon nanotube (CNT) field effect transistor (FET) was functionalized with human olfactory receptor 1A2 (hOR1A2)-embedded NDs (hOR1A2NDs). The hOR1A2NDs responded to rose scent molecules specifically, which were monitored electrically using the underlying CNT-FET. This strategy allowed us to quantitatively assess the contents of geraniol and citronellol, the main components of a rose scent, as low as 1 fM and 10 fM, respectively. In addition, it enabled us to selectively discriminate a specific rose odorant from other odorants. Significantly, we also demonstrated that the responses of hOR1A2NDs to a rose scent could be strongly enhanced by enhancer materials like a human nose. Furthermore, the method provided a means to quantitatively evaluate rose scent components in real samples such as rose oil. Since our method allows one to quantitatively evaluate general rose scent ingredients just like a human nose, it could be a powerful strategy for versatile basic research and various applications such as fragrance development.

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Citations

Mar 21, 2020·Biosensors·Jonathan D Bohbot, Sefi Vernick
Jan 25, 2020·Science China. Life Sciences·Dongseok MoonTai Hyun Park
Mar 25, 2019·International Journal of Molecular Sciences·Hyunil RyuTae-Joon Jeon
Oct 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Romain DurouxEstelle Loing
Jun 17, 2021·Lab on a Chip·Yusuke HirataShoji Takeuchi
Jan 7, 2022·ACS Applied Materials & Interfaces·Jin-Young JeongSeunghun Hong

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