PMID: 6397229Dec 18, 1984Paper

Human liver alcohol dehydrogenase isozymes: reduction of aldehydes and ketones

Biochemistry
J S DeetzB L Vallee

Abstract

The class I (alpha, beta 1, gamma 1, and gamma 2), II (pi), and III (chi) isozymes of human liver alcohol dehydrogenase (ADH) were isolated as electrophoretically homogeneous preparations to examine their kinetics of aldehyde and ketone reduction. While the oxidation of a wide variety of alcohols by ADH has been investigated extensively, the reduction of aldehydes and ketones has received much less attention even though the equilibrium favors the latter process. For each isozyme, the Km and kcat values were measured at pH 7.0 with acetaldehyde, pentanal, octanal, benzaldehyde, and cyclohexanone as substrates. Activity could not be detected with succinic semialdehyde and betaine aldehyde for any of the isozymes. The nonenzymatic hydration, oxidation, and aldol condensation of aldehydes in aqueous solutions present serious experimental obstacles in determining the isozymes' kinetic constants. The effects of these reactions on the enzymatic parameters were studied and compensated for. Michaelis constants for all class I and II isozymes vary by more than 8000-fold, from less than 1 microM for beta 1 gamma 1 and beta 1 beta 1 with octanal to 8.3 mM for pi-ADH for acetaldehyde. However, with any given aldehyde, these values vary by l...Continue Reading

References

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Citations

Jan 1, 1990·Pharmacology & Therapeutics·D P Agarwal, H W Goedde
Aug 6, 1991·Biochimica Et Biophysica Acta·Y W HoW K Ho
Feb 26, 1998·Alcohol·W E Lands
Apr 14, 2012·Environmental Science & Technology·Alessandra PirovanoA Jan Hendriks
May 21, 1999·European Journal of Biochemistry·S SvenssonJ O Höög
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T B BeisswengerB L Vallee
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G MårdhB L Vallee
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G MårdhB L Vallee
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A KumarJ Samuelson
Jan 19, 2006·Critical Reviews in Toxicology·Peter J O'BrienNandita Shangari
Jan 8, 1999·Journal of Enzyme Inhibition·S TrivićB M Anderson
Sep 30, 1996·FEBS Letters·S SvenssonJ O Höög
Mar 8, 2016·Oxidative Medicine and Cellular Longevity·José A HernándezAdela Rendón-Ramírez
Feb 3, 2009·Alcoholism, Clinical and Experimental Research·Jennifer R ChaseDavid A Fell
Apr 1, 1989·Analytical Biochemistry·J WierzchowskiB L Vallee
Aug 30, 1985·Biochemical and Biophysical Research Communications·A L DingleyB L Vallee

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