Human low-molecular-weight salivary mucin expresses the sialyl lewisx determinant and has L-selectin ligand activity

Biochemistry
Akraporn PrakobpholSusan J Fisher

Abstract

Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its function. Purified MG2 (Mr 120 000) was digested with trypsin, and the resulting Mr 90 000 fragment, which carried primarily O-linked oligosaccharides, was subjected to reductive beta-elimination. The released oligosaccharides were characterized by using nuclear magnetic resonance spectroscopy and mass spectrometry. Of the 41 different structures we detected, the most prominent included NeuAcalpha2-->3Galbeta1-->3GalNAc-ol (sialyl-T antigen), Galbeta1-->4(Fucalpha1-->3)GlcNAcbeta1-->6(Galbeta1 -->3)GalNAc-ol [type 2 core with Lewisx (Lex) determinant], and NeuAcalpha2-->3Galbeta1-->4(Fucalpha1-->3)GlcNAcbet a1-->6(Galbeta1--> 3) GalNAc-ol [type 2 core with sialyl Lex (sLex) determinant]. We also detected di-, tri-, and pentasaccharides with one sulfate group. Lex, sLex, and related sulfated structures are ligands for selectins, adhesion molecules that mediate leukocyte trafficking. Therefore, we investigated whether MG2...Continue Reading

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Citations

May 4, 2011·Clinical Oral Investigations·Rodrigo V SoaresElton G Zenóbio
Jul 17, 2007·Journal of Assisted Reproduction and Genetics·Russell A FoulkAkraporn Prakobphol
Dec 5, 2008·Glycobiology·Gustaf E RydellGöran Larson
Dec 25, 2008·Glycobiology·Halina Miller-PodrazaKarl-Anders Karlsson
Feb 24, 2006·Infection and Immunity·Daisuke TakamatsuPaul M Sullam
Mar 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshie IwaiHisashi Narimatsu
Feb 18, 2004·Mass Spectrometry Reviews·Joseph Zaia
Jun 9, 2015·Clinical Oral Investigations·Mikael ZadNiclas G Karlsson
Aug 26, 2006·Developmental Biology·Akraporn PrakobpholSusan J Fisher
Mar 11, 2017·Journal of Proteome Research·Steven C HallSusan J Fisher
Jul 27, 2007·Journal of Dental Research·E J Helmerhorst, F G Oppenheim
Sep 4, 2003·The Journal of Biological Chemistry·Catherine RobbeJean-Claude Michalski
Nov 23, 2017·Molecular & Cellular Proteomics : MCP·Zsuzsanna Darula, Katalin F Medzihradszky
Apr 9, 2005·Glycobiology·Kristina A ThomssonNiclas G Karlsson
Apr 9, 2002·Oral Diseases·A V Nieuw Amerongen, E C I Veerman

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