Abstract
Considering that in the allogeneic situation the adhesion of recipient lymphocytes to donor endothelial cells initiates the cellular rejection, we questioned the possible occurrence of a similar process in the xenogeneic situation. The adhesion of human peripheral blood lymphocytes (PBL) to porcine aortic endothelial cells (PAEC) was thus studied in an in vitro porcine-to-human xenogeneic model. It was found that 25.9% of human PBL adhered to resting PAEC. Furthermore, this adhesion increased significantly when the PAEC were stimulated by the human cytokine TNF-alpha (tumor necrosis factor-alpha). The effect of human TNF-alpha was concentration- and time-dependent and was maximal (from 25.9% to 35.6%) with 100 U/ml during 6 h. Moreover, blocking experiments with monoclonal antibody (mAb) demonstrated the role of the PBL adhesion molecules LFA-1 and especially VLA-4. Indeed, an anti-CD11a mAb decreased PBL adhesion to resting PAEC by 17.1% and to TNF-alpha stimulated PAEC by 16.9%, whereas an anti-CD49d mAb decreased dramatically PBL adhesion to resting PAEC by 53.1% and to TNF-alpha stimulated PAEC by 41.0%. Finally, phenotypic analysis of the adherent PBL showed that 50.5% of adherent cells to resting PAEC were NK (natural kil...Continue Reading
References
Dec 1, 1976·Transplantation·D H SachsS Rosenberg
Dec 1, 1978·In Vitro·S M Schwartz
Oct 1, 1992·Scandinavian Journal of Immunology·P GaléaP Bardos
Sep 1, 1991·Transplantation·A P DalmassoF H Bach
Oct 1, 1991·Current Opinion in Immunology·I V Hutchinson
Jul 25, 1990·Nucleic Acids Research·R DijkmansB Alfons
Jun 1, 1990·Transplantation·H TilgC Huber
Aug 1, 1990·Transplantation·D K ImagawaP J Levy
Sep 1, 1988·European Journal of Immunology·C PitzalisG Panayi
Nov 1, 1988·Cellular Immunology·D E CavenderM Ziff
Oct 1, 1987·The Journal of Experimental Medicine·C P Maury, A M Teppo
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeC R Minick
Dec 1, 1984·The Journal of Cell Biology·J C VoytaB R Zetter
Mar 27, 1995·Transplantation·I ValléeP Bardos
Sep 1, 1994·Transplant Immunology·H C PleassJ A Kirby
Jan 1, 1993·Transplant Immunology·P F HalloranJ Madrenas
Feb 1, 1993·Transplantation·M W HoffmannR Pichlmayr
Jul 1, 1993·Transplantation·A AikawaR A Sells
Feb 1, 1993·Endocrinology·L PitzelW Wuttke
Apr 1, 1993·Transplantation·D MorelB Bloch
Dec 1, 1996·Transplant Immunology·H WatierP Bardos
Citations
Jan 3, 2001·Transplantation Proceedings·A SarmentoD Machado
Aug 3, 2002·Immunology·Yasser E A ShaheinJosé M Pérez de la Lastra
Jul 6, 2001·Scandinavian Journal of Immunology·M K SchneiderJ D Seebach
Jul 29, 1998·Transplantation·S YunJ W Fabre
Feb 7, 2001·Transplantation·S A RushworthS Thompson
Mar 22, 2002·Transplantation·Mårten K J SchneiderJörg D Seebach
May 23, 1998·Annual Review of Immunology·H Auchincloss, D H Sachs
Feb 18, 2003·Xenotransplantation·Christine BonenfantHervé Watier
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Foy N OsborneRyszard T Smolenski
Feb 8, 2018·Journal of Immunology Research·Gisella Puga YungJörg D Seebach
Feb 17, 2019·Xenotransplantation·Yanli ZhaoHanchao Gao
Jan 1, 2016·Transplantation·Driss EhirchiouJörg D Seebach
Jul 29, 2015·Xenotransplantation·Wolf RamackersMichael Winkler